Lymphocyte Subset Panel 6 - Total Lymphocyte Enumeration with CD45RA and CD45RO
Ordering Recommendation

Useful for assessing primary T-cell immunodeficiency disorders.

Quantitative Flow Cytometry
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA), pink (K2EDTA), or green (sodium or lithium heparin). Hemogard tubes are preferred for laboratory automation and safety. 
Specimen Preparation
Transport 4 mL whole blood. (Min: 0.5 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Clotted or hemolyzed specimens. 
Specimens must be analyzed within stability times provided. Some medication may affect immunophenotyping results and should be provided on the patient test request form. 
EDTA: Ambient: 72 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Heparin: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: EDTA:
Ambient: 30 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Heparin: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
Effective February 20, 2018

Reports include age appropriate reference intervals and interpretation.

Pediatric reference values (0 - 6 days up to 10 - 15 years) taken from Scandinavian Journal of Immunology 2012; 75, 436-444.
Adult and Geriatric (16 - 64 and 65 plus years) ranges were developed in-lab.
Test Number
0-6 days
1 week-1 month
2-4 months
5-8 months
9-14 months
15-23 months
2-4 years
5-9 years
10-15 years
16-64 years
65 years or older
% CD338-88%55-90 %49-97 %49-95%56-87%36-92%52-92%55-97%52-90%62-87%62-89%
1400-6800 cells/µL1900-8400 cells/µL2200-9200 cells/µL1400-11500 cells/µL2400-8300 cells/µL700-8800 cells/µL850-4300 cells/µL770-4000 cells/µL850-3200 cells/µL570-2400 cells/µL660-2200 cells/µL
% CD426-62 %39-69 %37-69 %27-81%25-86%16-91%25-66%26-61%20-65%32-64%35-68%
1000-4800 cells/µL1500-6000 cells/µL1600-6500 cells/µL1000-7200 cells/µL1300-7100
400-7200 cells/µL500-2700 cells/µL400-2500 cells/µL400-2100 cells/µL430-1800 cells/µL490-1600 cells/µL
% CD45RA60-100%63-100%66-100%68-99%68-98%57-100%53-96%47-97%39-93%28-71%19-62%
Absolute CD45RA900-4500 cells/µL1100-5200 cells/µL1200-5300 cells/µL800-5900 cells/µL900-5200 cells/µL400-5600 cells/µL380-2500 cells/µL250-2000 cells/µL230-1400 cells/µL150-870 cells/µL260-1000 cells/µL
% CD45RO2-44%2-36%1-42%1-46%4-29%5-39%11-50%8-76%18-68%28-72%38-81%
Absolute CD45RO98-1300 cells/µL110-1200 cells/µL90-1400 cells/µL100-950 cells/µL160-710 cells/µL68-630 cells/µL150-640 cells/µL100-510 cells/µL160-700 cells/µL190-1050 cells/µL490-1200 cells/µL
% CD85-37%7-35%6-41%10-35%7-58%7-40%9-49%13-47%14-40%15-46%10-46%
200-2700 cells/µL300-2700
300-3400 cells/µL200-5400 cells/µL400-4100 cells/µL200-2800 cells/µL200-1800 cells/µL200-1700 cells/µL300-1300 cells/µL210-1200 cells/µL150-1050 cells/µL
CD4:CD8 Ratio1.00-2.601.30-6.301.70-3.901.60-3.801.30-3.900.90-3.700.90-2.900.90-2.600.90-3.400.80-3.900.80-6.17
% CD193-30%3-60%8-33%4-54%3-77%8-45%8-39%4-33%7-24%6-23%5-21%
140-2000 cells/µL180-3500 cells/µL520-2300 cells/µL130-6300 cells/µL110-7700 cells/µL160-3700 cells/µL180-1300 cells/µL100-800 cells/µL120-740 cells/µL91-610 cells/µL74-510 cells/µL
% NK-cells8-62%3-23%2-20%2-36%1-64%1-96%2-25%2-31%4-51%4-26%5-28%
Absolute NK-cells500-3100 cells/µL140-1900 cells/µL97-2000 cells/µL68-3900 cells/µL71-3500 cells/µL55-4000 cells/µL61-510 cells/µL70-590 cells/µL92-1200 cells/µL78-470 cells/µL74-620 cells/µL

Interpretive Data
The CD4 cells are Helper T-cells expressing both CD3 and CD4. The CD8 cells are Cytotoxic T-cells expressing both CD3 and CD8. The B-cells express CD19 but not CD3. The NK-cells express either CD16 or CD56 (or both) but not CD3. CD3, CD4, CD8, CD19 and NK-cell percentages are reported as a percent of total lymphocytes. The CD45RA cells express both CD4 and the "naive" CD45RA antigens while CD45RO cells express both CD4 and the CD45RO "memory" antigens. CD45RA and CD45RO percentages are reported as a percent of total CD4 cells. CD4 T-cell levels are a criterion for categorizing HIV-related clinical conditions by the CDC's classification system for HIV infection. The measurement of CD4 T-cell levels has been used to establish decision points for initiating P. jirovecii prophylaxis, antiviral therapy and to monitor the efficacy of treatment. The Public Health Service (PHS) has recommended that CD4 T-cell levels be monitored every 3 to 6 months in all HIV-infected persons.

Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

This test is designed for enumerating the percent and absolute cell count of lymphocyte subsets in lysed whole blood. Whole blood is added to fluorochrome-labeled antibodies that bind specifically to cell surface antigens on lymphocytes. After incubation, lysing and fixation, percent and absolute count are enumerated utilizing an internal quantitation standard. Additional CBC data is not required.

During HIV infection, antiviral therapy is often initiated when the absolute CD4 count drops below 500 cells/µL. When the absolute CD4 count drops below 200 cells/µL, therapeutic prophylaxis against PCP and other opportunistic infections may be initiated. When the absolute CD4 count drops below 100 cells/µL, prophylaxis against Mycobacterium avium complex is recommended.

The CD45RA subset delineates a "naive" CD4 T-cell population. The CD45RO subset delineates a "memory" CD4 T-cell population. With disease progression in HIV-infected individuals, a decrease in CD45RA "naive" cells may be observed.

For longitudinal studies involving serial monitoring, specimen collections should be performed at the same time of day.
Hotline History
View Hotline History
CPT Code(s)
86355; 86357; 86359; 86360; 86356 x2
Component Test Code*Component Chart NameLOINC
0095617Lymphocyte Subset Panel 6 Information48767-8
0095700Absolute CD1915195-1
0095701% Natural Killer Cells32519-1
0095702Absolute Natural Killer Cells20604-5
0095751Absolute CD45RO34475-4
0095752% CD45RO41994-5
0095816Absolute CD45RA26759-1
0095827% CD45RA17157-9
0095902Absolute CD38122-4
0095903% CD320599-7
0095906Absolute CD424467-3
0095911Absolute CD814135-8
2012857% CD48123-2
2012858% CD832518-3
2012859% CD1920593-0
2012860CD4:CD8 Ratio54218-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • CD3, CD4, CD45RA, CD45RO, CD8, CD19 and NK-cells
  • Helper T-Cell count
  • Immune Status-Flow Cytometry
  • P. jiroveci prophylaxis determination
  • T-Cell Immunodeficiency Extended Profile