- Patient Preparation
- Breast tumor tissue.
- Specimen Preparation
- Paraffin embed tissue block enriched with breast tumor. If no normal tissue is included in the block, supply a control tissue block to be processed in parallel.
- Storage/Transport Temperature
- Unacceptable Conditions
- No tumor tissue remaining on block. Specimens fixed in Bouin's solution (picric acid), mercuric chloride containing fixatives (e.g., B5, Zenker's solution), or ethanol-based fixatives containing ethylene glycol, acetic acid, or zinc chloride. Decalcified specimens.
- Include H&E stained slide and surgical pathology report.
- Paraffin block: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
The tumor-specific S-phase is used when possible. An average histogram S-phase is used for diploid and some aneuploid tumors where the tumor and host S-phases cannot be separated. An average histogram S-phase is also used when the percentage of aneuploid cells in the histogram is low (less than 25%). The S-phase interpretation is based on comparison to over 5,000 breast tumors, controlled for histogram type (diploid, near-diploid, aneuploid) and sample type (fresh/frozen, paraffin). The appropriate population is divided into thirds and compared to the sample S-phase for an interpretation of low, moderate, or high S-phase.
|Component Test Code*||Component Chart Name||LOINC|
|0095731||DNA Content, Breast||30911-2|
|0095732||DNA Content, Breast, S-Phase Interp.||41294-0|
|2008900||DNA Index, Breast|
- Breast Cancer aneuploidy
- DNA Ploidy and S-Phase