Human Immunodeficiency Virus 1 (HIV-1) by Qualitative PCR
0093061
 
Ordering Recommendation
Mnemonic
HIV DNA
Methodology
Qualitative Polymerase Chain Reaction
Performed
Mon-Sat
Reported
2-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Do not freeze specimen. Transport 1 mL whole blood. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Heparinized specimens. Frozen specimens.  
Remarks
 
Stability
Ambient: 12 hours; Refrigerated: 5 days; Frozen: Unacceptable  
Reference Interval
Not detected
Interpretive Data
This test detects human immunodeficiency virus type 1 (HIV-1) DNA and RNA. The assay methodology is polymerase chain reaction (PCR) using the COBAS AmpliPrep/COBAS TaqMan HIV-1 Qual Test. This test is optimized to yield equivalent amplification of Group M subtypes of HIV-1.

A result of "Not Detected" does not rule out HIV-1 nucleic acid concentrations below the limit of detection of the assay or the presence of PCR inhibitors in the patient specimen. Improper specimen handling can cause false negatives. PCR may not detect infection in the first months of life. The diagnosis of HIV-1 infection should be made based on clinical presentation and results from additional diagnostic tests. Diagnosis should not be made based solely on a single HIV-1 test. False positives can be caused by PCR contamination.

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).


See Compliance Statement D: www.aruplab.com/CS
Note
CPT Code(s)
87535
Components
Component Test Code*Component Chart Name
0093062HIV-1 PCR, Qualitative
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • HIV 1 DNA and RNA
  • HIV Qualitative
  • HIV-1 DNA
  • HIV-1 proviral DNA
  • HIV1 detection
  • Nucleic Acid Amplification Test (NAAT)