Nicotine and Metabolites - Confirmation/Quantitation, Serum or Plasma
Ordering Recommendation
Preferred test for documenting nicotine cessation over time. Use to detect and monitor nicotine, cotinine, and trans-3'-hydroxycotinine in serum or plasma.
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Plain red, green (sodium heparin), lavender (EDTA), pink (K2EDTA), or SST.  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 4 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma or whole blood collected in lt. blue (sodium citrate). Specimens exposed to repeated freeze/thaw cycles.  
After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 3 years  
Reference Interval
Interpretive Data
Drugs and analytes covered: nicotine, cotinine (metabolite), 3-OH-cotinine (metabolite).

Positive cutoff:
2 ng/mL

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

For medical purposes only; not valid for forensic use.

See Compliance Statement B:
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
80323; (Alt code: G6055)
Component Test Code*Component Chart NameLOINC
0092362Nicotine, Serum or Plasma3853-9
0092363Cotinine, Serum or Plasma10365-5
00923643-OH-Cotinine, Serum or Plasma35140-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 3-Hydroxycotinine
  • Cotinine
  • Nicotine
  • Nicotine and Cotinine, LC/MS/MS, Serum/Plasma
  • Pain Management