Nicotine and Metabolites, Urine
Ordering Recommendation
Assess active exposure to nicotine and compliance with smoking-cessation programs. Use to confirm cotinine screening results.
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Random urine.  
Specimen Preparation
Transfer 4 mL with no additives or preservatives urine to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Room temperature.  
Unacceptable Conditions
Specimens exposed to repeated freeze/thaw cycles.  
Ambient: 10 days; Refrigerated: 10 days; Frozen: 8 months  
Reference Interval
 Unexposed non-​tobacco user Passive exposure Abstinent user for greater than 2 weeks Active tobacco product use
Nicotine Less than 2 ng/mL Less than 20 ng/mL Less than 30 ng/mL 1000-​5000 ng/mL
Cotinine Less than 5 ng/mL Less than 20 ng/mL Less than 50 ng/mL 1000-​8000 ng/mL
3-​OH-​Cotinine Less than 50 ng/mL Less than 50 ng/mL Less than 120 ng/mL 3000-​25000 ng/mL
Nornicotine Less than 2 ng/mL Less than 2 ng/mL Less than 2 ng/mL 30-​900 ng/mL
Anabasine Less than 3 ng/mL Less than 3 ng/mL Less than 3 ng/mL 3-​500 ng/mL*
Adapted from Clinical Chemistry 2002;48:1460-​71.
Interpretive Data
The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

For medical purposes only; not valid for forensic use.

See Compliance Statement B:  
CPT Code(s)
Component Test Code*Component Chart Name
0092357Nicotine, Urine
0092358Cotinine, Urine
00923593-OH-Cotinine, Urine
0092360Nornicotine, Urine
0092365Anabasine, Urine
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 3-Hydroxycotinine
  • Anabasine
  • Cotinine
  • Nicotine and Cotinine, LC/MS/MS, Urine
  • Nornicotine
  • Pain Management