Nicotine and Metabolites - Confirmation/Quantitation, Urine
0092356
Ordering Recommendation
Assess active exposure to nicotine and compliance with smoking-cessation programs. Use to confirm cotinine screening results.
Mnemonic
NICOTINEUR
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Performed
Sun-Sat
Reported
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Random urine.  
Specimen Preparation
Transfer 4 mL with no additives or preservatives urine to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Room temperature.  
Unacceptable Conditions
Specimens exposed to repeated freeze/thaw cycles.  
Remarks
  
Stability
Ambient: 10 days; Refrigerated: 10 days; Frozen: 8 months  
Reference Interval
   
Interpretive Data
Methodology: Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Drug and analytes covered
: nicotine, cotinine (metabolite), 3-OH-cotinine (metabolite), nornicotine (metabolite),anabasine (tobacco biomarker). All drug and metabolites are the non-glucuronidated (free) form.

Positive cutoff
:
Nicotine.................2 ng/mL
Cotinine.................5 ng/mL
3-OH-Cotinine......50 ng/mL
Nornicotine...........2 ng/mL
Anabasine.............3 ng/mL

The absence of expected drug(s) and/or drug metabolite(s) may indicate non-compliance, inappropriate timing of specimen collection relative to drug administration, poor drug absorption, diluted/adulterated urine, or limitations of testing. The concentration value must be greater than or equal to the cutoff to be reported as positive. Interpretive questions should be directed to the laboratory.

See Compliance Statement B: www.aruplab.com/CS  
Note
 
CPT Code(s)
83887
Components
Component Test Code*Component Chart Name
0092357Nicotine, Urine
0092358Cotinine, Urine
00923593-OH-Cotinine, Urine
0092360Nornicotine, Urine
0092365Anabasine, Urine
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 3-Hydroxycotinine
  • Anabasine
  • Cotinine
  • Nicotine and Cotinine, LC/MS/MS, Urine
  • Nornicotine
  • Pain Management