Pregnenolone by LC-MS/MS, Serum or Plasma
Ordering Recommendation
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube. Also acceptable: Plain red, lavender (EDTA), pink  (K2EDTA), or green (sodium or lithium heparin).  
Specimen Preparation
Specimen Preparation: Separate serum or plasma cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum or plasma in two ARUP Standard Transport Tubes and freeze immediately. (Min: 0.25 mL/container)  
Storage/Transport Temperature
CRITICAL FROZEN. Additional specimens must be submitted when multiple tests are ordered.  
Unacceptable Conditions
Refrigerated or room temperature specimens.  
After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 6 months  
Reference Interval
Age Female Male
6-​12 months 13-​327 ng/dL 13-​327 ng/dL
13-​23 months 12-​171 ng/dL 12-​171 ng/dL
2-​4 years 15-​125 ng/dL 10-​125 ng/dL
5-​6 years 13-​191 ng/dL 10-​156 ng/dL
7-​9 years 14-​150 ng/dL 13-​205 ng/dL
10-​12 years 19-​220 ng/dL 15-​151 ng/dL
13-​15 years 22-​210 ng/dL 18-​197 ng/dL
16-​17 years 22-​229 ng/dL 17-​228 ng/dL
18 years and older 15-​132 ng/dL 23-​173 ng/dL
Tanner Stage I 15-​171 ng/dL 13-​156 ng/dL
Tanner Stage II 22-​229 ng/dL 12-​143 ng/dL
Tanner Stage III 34-​215 ng/dL 16-​214 ng/dL
Tanner Stage IV-​V 26-​235 ng/dL 19-​201 ng/dL
Interpretive Data

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.