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CBFB-MYH11, inv(16) by RT-PCR (INACTIVE as of 11/17/14. Refer to 2011114)
0092209
Ordering Recommendation
Mnemonic
INV(16)
Methodology
Reverse Transcription Polymerase Chain Reaction
Performed
Varies
Reported
4-7 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or bone marrow (EDTA). 
Specimen Preparation
Transport 5 mL whole blood or 3 mL bone marrow. (Min: 1 mL whole blood or 1 mL bone marrow) Specimens must be received within 48 hours of collection due to lability of RNA. 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Serum or plasma. Frozen or clotted specimens. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed specimens. 
Remarks
 
Stability
Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
CBFB (core binding factor β-chain)-MYH11 (myosin heavy chain 11 gene) fusion transcripts are detected in approximately 10% of de novo acute myelogenous leukemias (AML).  One half of these cases belong to AML subtype M4 with abnormal eosinophils (AML-M4Eo).  The fusion results from a pericentric inversion on chromosome 16 inv(16)(p13q22) or rarely from the translocation t(16;16)(p13;q22).  Based on the breakpoints in the CBFB and MYH11 genes, greater than 95% of analyzed inv(16) cases belong to three types: A (88%), D (5%), and E (5%).
CPT Code(s)
81401
Components
Component Test Code*Component Chart NameLOINC
0096364CBFB-MYH11, inv(16) by RT-PCR70291-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • CBFB-MYH11 inv(16) fusion gene
  • CBFB-MYH11 Inversion
  • inv(16)