Tacrolimus by Tandem Mass Spectrometry
0090612
Ordering Recommendation
 
Mnemonic
TACRO
Methodology
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed
Sun-Sat
Reported
Within 24 hours  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Pre-dose (trough) levels should be drawn.  
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 1 mL whole blood. (Min: 0.25 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens.  
Remarks
  
Stability
Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 2 months  
Reference Interval
Effective February 18, 2014
 
 Therapeutic Range:
Kidney transplant: 0-​3 months post-​transplant: 7.0-​20.0 ng/mL
3 months and older: 5.0-​15.0 ng/mL
Heart transplant: 0-​3 months post-​transplant: 10.0-​20.0 ng/mL
3 months and older: 5.0-​15.0 ng/mL
Liver transplant: 1-​12 months post-​transplant: 5-​20 ng/mL
Toxic value: Greater than 25 ng/mL
Interpretive Data
Therapeutic range is based on a whole blood specimen drawn 12 hours post-dose or prior to next dose (the trough). The optimal therapeutic range for a given patient may differ from this suggested range based on the indication for therapy, treatment phase (initiation or maintenance), use in combination with other drugs, time of specimen collection relative to prior dose, type of transplanted organ, and/or the therapeutic approach of the transplant center.

See Compliance Statement B: www.aruplab.com/CS  
Note
Tacrolimus (Prograf) whole blood concentrations can be measured by either chromatographic or immunoassay methodologies. These two methodologies are not directly interchangeable, and the measured tacrolimus whole blood concentration depends on the methodology used. Reference ranges may vary according to the specific immunoassay or HPLC-MS/MS test. Generally, immunoassays have been reported to have a positive bias relative to HPLC-MS/MS assays due to the detection of antibody cross-reactivity with tacrolimus metabolites. 
CPT Code(s)
80197
Components
Component Test Code*Component Chart Name
0090612Tacrolimus by HPLC-MS/MS
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • FK506
  • Prograf
  • Prograf{R} (Tacrolimus by Tandem Mass Spectrometry)
  • Protopic