Testosterone Free, Females or Children
Ordering Recommendation
Most sensitive test for detection of hyperandrogenemia in women and children. Acceptable test for androgen deficiency in men.
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
Total Testosterone and SHBG are measured and free testosterone is estimated from these measurements.
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Collect between 6-10 a.m.  
Serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL)  
Storage/Transport Temperature
Unacceptable Conditions
EDTA plasma.  
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.  
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
Female Free Testosterone, pg/mL Male Free Testosterone, pg/mL
1-​6 years: Less than 0.6 pg/mL
7-​9 years: 0.6-​1.8 pg/mL
10-​11 years: 0.1-​3.5 pg/mL
12-​13 years: 0.9-​6.8 pg/mL
14-​15 years: 1.2-​7.5 pg/mL
16-​17 years: 1.2-​9.9 pg/mL
18-​30 years: 0.8-​7.4 pg/mL
31-​40 years: 1.3-​9.2 pg/mL
41-​51 years: 1.1-​5.8 pg/mL
Postmenopausal: 0.6-​3.8 pg/mL
Tanner Stage I: Less than 2.2 pg/mL
Tanner Stage II: 0.4-​4.5 pg/mL
Tanner Stage III: 1.3-​7.5 pg mL
Tanner Stage IV: 1.1-​15.5 pg/mL
Tanner Stage V: 0.8-​9.2 pg/mL
1-​6 years: Less than 0.6 pg/mL
7-​9 years: 0.1-​0.9 pg/mL
10-​11 years: 0.1-​6.3 pg/mL
12-​13 years: 0.5-​98.0 pg/mL
14-​15 years: 3-​138.0 pg/mL
16-​17 years: 38.0-​173.0 pg/mL
18 years and older: 47-​244 pg/mL
Tanner Stage I: Less than or equal to 3.7 pg/mL
Tanner Stage II: 0.3-​21 pg/mL
Tanner Stage III: 1.0-​98.0 pg mL
Tanner Stage IV: 35.0-​169.0 pg/mL
Tanner Stage V: 41.0-​239.0 pg/mL
Interpretive Data
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to SHBG.

See Compliance Statement B: www.aruplab.com/CS  
This test is suggested for women and children due to the improved sensitivity of testosterone by LC-MS/MS. Testosterone Free, Females and Children by tandem mass spectrometry, only provides a result for the free testosterone value (pg/mL).
CPT Code(s)
Component Test Code*Component Chart Name
0081096Testosterone, Free LC-MS/MS
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.