Testosterone Free, Females or Children
Ordering Recommendation
Most sensitive test for detection of hyperandrogenemia in women and children. Acceptable test for androgen deficiency in men.
Submit With Order
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
Total Testosterone and SHBG are measured and free testosterone is estimated from these measurements.
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect between 6-10 a.m.  
Serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL)  
Storage/Transport Temperature
Unacceptable Conditions
EDTA plasma.  
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.  
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
Female Free Testosterone, pg/mL Male Free Testosterone, pg/mL
1-​6 years: Less than 0.6 pg/mL
7-​9 years: 0.6-​1.8 pg/mL
10-​11 years: 0.1-​3.5 pg/mL
12-​13 years: 0.9-​6.8 pg/mL
14-​15 years: 1.2-​7.5 pg/mL
16-​17 years: 1.2-​9.9 pg/mL
18-​30 years: 0.8-​7.4 pg/mL
31-​40 years: 1.3-​9.2 pg/mL
41-​51 years: 1.1-​5.8 pg/mL
Postmenopausal: 0.6-​3.8 pg/mL
Tanner Stage I: Less than 2.2 pg/mL
Tanner Stage II: 0.4-​4.5 pg/mL
Tanner Stage III: 1.3-​7.5 pg mL
Tanner Stage IV: 1.1-​15.5 pg/mL
Tanner Stage V: 0.8-​9.2 pg/mL
1-​6 years: Less than 0.6 pg/mL
7-​9 years: 0.1-​0.9 pg/mL
10-​11 years: 0.1-​6.3 pg/mL
12-​13 years: 0.5-​98.0 pg/mL
14-​15 years: 3-​138.0 pg/mL
16-​17 years: 38.0-​173.0 pg/mL
18 years and older: 47-​244 pg/mL
Tanner Stage I: Less than or equal to 3.7 pg/mL
Tanner Stage II: 0.3-​21 pg/mL
Tanner Stage III: 1.0-​98.0 pg mL
Tanner Stage IV: 35.0-​169.0 pg/mL
Tanner Stage V: 41.0-​239.0 pg/mL
Interpretive Data
The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to SHBG.

See Compliance Statement B: www.aruplab.com/CS
This test is suggested for women and children due to the improved sensitivity of testosterone by LC-MS/MS. Testosterone Free, Females and Children by tandem mass spectrometry, only provides a result for the free testosterone value (pg/mL).
CPT Code(s)
Component Test Code*Component Chart Name
0081096Testosterone, Free LC-MS/MS
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References