Testosterone, Females or Children
Ordering Recommendation
Use in conjunction with free testosterone in the evaluation of suspected hyperandrogenemia in women and children.
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Collect between 6-10 a.m.  
Serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.2 mL)  
Storage/Transport Temperature
Unacceptable Conditions
EDTA plasma.  
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.  
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
Effective August 19, 2013
Age Female Male
Premature (26-​28 weeks) 5-​16 ng/dL 59-​125 ng/dL
Premature (31-​35 weeks) 5-​22 ng/dL 37-​198 ng/dL
Newborn 20-​64 ng/dL 75-​400 ng/dL
1-​5 months Less than 20 ng/dL 14-​363 ng/dL
6-​24 months Less than 9 ng/dL Less than 37 ng/dL
2-​3 years Less than 20 ng/dL Less than 15 ng/dL
4-​5 years Less than 30 ng/dL Less than 19 ng/dL
6-​7 years Less than 7 ng/dL Less than 13 ng/dL
8-​9 years 1-​11ng/dL 2-​8 ng/dL
10-​11 years 3-​32 ng/dL 2-​165 ng/dL
12-​13 years 6-​50 ng/dL 3-​619 ng/dL
14-​15 years 6-​52 ng/dL 31-​733 ng/dL
16-​17 years 9-​58 ng/dL 158-​826 ng/dL
18-​39 years 9-​55 ng/dL 300-​1080 ng/dL
40-​59 years 9-​55 ng/dL 300-​890 ng/dL
60 years and older 5-​32 ng/dL 300-​720 ng/dL
Premenopausal (Greater than 18 years) 9-​55 ng/dL Does Not Apply
Postmenopausal 5-​32 ng/dL Does Not Apply
Tanner Stage I 2-​17 ng/dL 2-​15 ng/dL
Tanner Stage II 5-​40 ng/dL 3-​303 ng/dL
Tanner Stage III 10-​63 ng/dL 10-​851 ng/dL
Tanner Stage IV-​V 11-​62 ng/dL 162-​847 ng/dL
Interpretive Data
Total testosterone values may not reflect optimal concentrations in all individuals. Free or bioavailable testosterone measurements may provide supportive information.

See Compliance Statement B: www.aruplab.com/CS  
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.
CPT Code(s)
Component Test Code*Component Chart Name
0081058Testosterone, LC-MS/MS
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Total Testosterone