Testosterone, Bioavailable and Sex Hormone Binding Globulin (Includes Total Testosterone), Females or Children
0081057
Ordering Recommendation
Acceptable test in the evaluation of suspected hyperandrogenemia in women and children. Acceptable test for evaluating androgen deficiency in men.
Mnemonic
BIO T MASS
Methodology
Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.
Performed
Sun-Sat
Reported
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Collect between 6-10 a.m.  
Collect
Serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
EDTA plasma.  
Remarks
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.  
Stability
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
 
 
Test Number Components Reference Interval
0099375Sex Hormone Binding Globulin
 
Age Male Female
1-​30 days
31-​364 days
1-​3 years
4-​6 years
7-​9 years
10-​12 years
13-​15 years
16-​17 years
18 years and older
13-​85 nmol/L
70-​250 nmol/L
50-​180 nmol/L
45-​175 nmol/L
28-​190 nmol/L
23-​160 nmol/L
13-​140 nmol/L
10-​60 nmol/L
11-​80 nmol/L
14-​60 nmol/L
60-​215 nmol/L
60-​190 nmol/L
55-​170 nmol/L
35-​170 nmol/L
17-​155 nmol/L
11-​120 nmol/L
19-​145 nmol/L
30-​135 nmol/L
Tanner Stage I
Tanner Stage II
Tanner Stage III
Tanner Stage IV
Tanner Stage V
26-​186 nmol/L
22-​169 nmol/L
13-​104 nmol/L
11-​60 nmol/L
11-​71 nmol/L
30-​173 nmol/L
16-​127 nmol/L
12-​98 nmol/L
14-​151 nmol/L
23-​165 nmol/L
 Testosterone LC-​MS, Bioavailable  
 
Female -​ Bioavailable Testosterone, ng/dL Male -​ Bioavailable Testosterone, ng/dL
1-​6 years: Less than 1.3 ng/dL
7-​9 years: 0.3-​5.0 ng/dL
10-​11 years: 0.4-​9.6 ng/dL
12-​13 years: 1.7-​18.8 ng/dL
14-​15 years: 3.0-​22.6 ng/dL
16-​17 years: 3.3-​28.6 ng/dL
18-​30 years: 2.2-​20.6 ng/dL
31-​40 years: 4.1-​25.5 ng/dL
41-​51 years: 2.8-​16.5 ng/dL
Postmenopausal: 1.5-​9.4 ng/dL
Tanner Stage I: 0.3-​5.5 ng/dL
Tanner Stage II: 1.2-​15.0 ng/dL
Tanner Stage III: 3.8-​28.0 ng/dL
Tanner Stage IV: 2.8-​39.0 ng/dL
Tanner Stage V: 2.5-​23.0 ng/dL
1-​6 years: Less than 1.3 ng/dL
7-​9 years: 0.3-​2.8 ng/dL
10-​11 years: 0.1-​17.9 ng/dL
12-​13 years: 1.4-​288.0 ng/dL
14-​15 years: 9.5-​337.0 ng/dL
16-​17 years: 35.0-​509.0 ng/dL
18 years and older: 130-​680 ng/dL
Tanner Stage I: 0.3-​13.0 ng/dL
Tanner Stage II: 0.3-​59.0 ng/dL
Tanner Stage III: 1.9-​296.0 ng/dL
Tanner Stage IV: 40.0-​485.0 ng/dL
Tanner Stage V: 124.0-​596.0 ng/dL
0081059Testosterone Free, Females or Children  
 
Female Free Testosterone, pg/mL Male Free Testosterone, pg/mL
1-​6 years: Less than 0.6 pg/mL
7-​9 years: 0.6-​1.8 pg/mL
10-​11 years: 0.1-​3.5 pg/mL
12-​13 years: 0.9-​6.8 pg/mL
14-​15 years: 1.2-​7.5 pg/mL
16-​17 years: 1.2-​9.9 pg/mL
18-​30 years: 0.8-​7.4 pg/mL
31-​40 years: 1.3-​9.2 pg/mL
41-​51 years: 1.1-​5.8 pg/mL
Postmenopausal: 0.6-​3.8 pg/mL
Tanner Stage I: Less than 2.2 pg/mL
Tanner Stage II: 0.4-​4.5 pg/mL
Tanner Stage III: 1.3-​7.5 pg mL
Tanner Stage IV: 1.1-​15.5 pg/mL
Tanner Stage V: 0.8-​9.2 pg/mL
1-​6 years: Less than 0.6 pg/mL
7-​9 years: 0.1-​0.9 pg/mL
10-​11 years: 0.1-​6.3 pg/mL
12-​13 years: 0.5-​98.0 pg/mL
14-​15 years: 3-​138.0 pg/mL
16-​17 years: 38.0-​173.0 pg/mL
18 years and older: 47-​244 pg/mL
Tanner Stage I: Less than or equal to 3.7 pg/mL
Tanner Stage II: 0.3-​21 pg/mL
Tanner Stage III: 1.0-​98.0 pg mL
Tanner Stage IV: 35.0-​169.0 pg/mL
Tanner Stage V: 41.0-​239.0 pg/mL
0081058Testosterone, Females or Children Effective August 19, 2013
 
Age Female Male
Premature (26-​28 weeks) 5-​16 ng/dL 59-​125 ng/dL
Premature (31-​35 weeks) 5-​22 ng/dL 37-​198 ng/dL
Newborn 20-​64 ng/dL 75-​400 ng/dL
1-​5 months Less than 20 ng/dL 14-​363 ng/dL
6-​24 months Less than 9 ng/dL Less than 37 ng/dL
2-​3 years Less than 20 ng/dL Less than 15 ng/dL
4-​5 years Less than 30 ng/dL Less than 19 ng/dL
6-​7 years Less than 7 ng/dL Less than 13 ng/dL
8-​9 years 1-​11ng/dL 2-​8 ng/dL
10-​11 years 3-​32 ng/dL 2-​165 ng/dL
12-​13 years 6-​50 ng/dL 3-​619 ng/dL
14-​15 years 6-​52 ng/dL 31-​733 ng/dL
16-​17 years 9-​58 ng/dL 158-​826 ng/dL
18-​39 years 9-​55 ng/dL 300-​1080 ng/dL
40-​59 years 9-​55 ng/dL 300-​890 ng/dL
60 years and older 5-​32 ng/dL 300-​720 ng/dL
Premenopausal (Greater than 18 years) 9-​55 ng/dL Does Not Apply
Postmenopausal 5-​32 ng/dL Does Not Apply
Tanner Stage I 2-​17 ng/dL 2-​15 ng/dL
Tanner Stage II 5-​40 ng/dL 3-​303 ng/dL
Tanner Stage III 10-​63 ng/dL 10-​851 ng/dL
Tanner Stage IV-​V 11-​62 ng/dL 162-​847 ng/dL
Interpretive Data
The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.

See Compliance Statement B: www.aruplab.com/CS  
Note
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS.
CPT Code(s)
84403; 84270
Components
Component Test Code*Component Chart Name
0081058Testosterone, LC-MS/MS
0081096Testosterone, Free LC-MS/MS
0081098Testosterone LC-MS, Bioavailable
0099375Sex Hormone Binding Globulin
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases