Testosterone, Bioavailable and Sex Hormone Binding Globulin (Includes Total Testosterone), Females or Children
Ordering Recommendation

Acceptable test in the evaluation of suspected hyperandrogenemia in women and children. Acceptable test for evaluating androgen deficiency in men.

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry/Electrochemiluminescent Immunoassay
The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect between 6-10 a.m. 
Serum separator tube or green (sodium or lithium heparin). 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.8 mL) 
Storage/Transport Temperature
Unacceptable Conditions
EDTA plasma. 
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS. 
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months 
Reference Interval
Test Number
Reference Interval
0099375Sex Hormone Binding Globulin
1-30 days
31-364 days
1-3 years
4-6 years
7-9 years
10-12 years
13-15 years
16-17 years
18 years and older
13-85 nmol/L
70-250 nmol/L
50-180 nmol/L
45-175 nmol/L
28-190 nmol/L
23-160 nmol/L
13-140 nmol/L
10-60 nmol/L
11-80 nmol/L
14-60 nmol/L
60-215 nmol/L
60-190 nmol/L
55-170 nmol/L
35-170 nmol/L
17-155 nmol/L
11-120 nmol/L
19-145 nmol/L
30-135 nmol/L
Tanner Stage I
Tanner Stage II
Tanner Stage III
Tanner Stage IV
Tanner Stage V
26-186 nmol/L
22-169 nmol/L
13-104 nmol/L
11-60 nmol/L
11-71 nmol/L
30-173 nmol/L
16-127 nmol/L
12-98 nmol/L
14-151 nmol/L
23-165 nmol/L

 Testosterone LC-MS, Bioavailable
Female - Bioavailable Testosterone, ng/dL
Male - Bioavailable Testosterone, ng/dL
1-6 years: Less than 1.3 ng/dL
7-9 years: 0.3-5.0 ng/dL
10-11 years: 0.4-9.6 ng/dL
12-13 years: 1.7-18.8 ng/dL
14-15 years: 3.0-22.6 ng/dL
16-17 years: 3.3-28.6 ng/dL
18-30 years: 2.2-20.6 ng/dL
31-40 years: 4.1-25.5 ng/dL
41-51 years: 2.8-16.5 ng/dL
Postmenopausal: 1.5-9.4 ng/dL
Tanner Stage I: 0.3-5.5 ng/dL
Tanner Stage II: 1.2-15.0 ng/dL
Tanner Stage III: 3.8-28.0 ng/dL
Tanner Stage IV: 2.8-39.0 ng/dL
Tanner Stage V: 2.5-23.0 ng/dL
1-6 years: Less than 1.3 ng/dL
7-9 years: 0.3-2.8 ng/dL
10-11 years: 0.1-17.9 ng/dL
12-13 years: 1.4-288.0 ng/dL
14-15 years: 9.5-337.0 ng/dL
16-17 years: 35.0-509.0 ng/dL
18 years and older: 130-680 ng/dL
Tanner Stage I: 0.3-13.0 ng/dL
Tanner Stage II: 0.3-59.0 ng/dL
Tanner Stage III: 1.9-296.0 ng/dL
Tanner Stage IV: 40.0-485.0 ng/dL
Tanner Stage V: 124.0-596.0 ng/dL

0081059Testosterone Free, Females or Children
Female Free Testosterone, pg/mL
Male Free Testosterone, pg/mL
1-6 years: Less than 0.6 pg/mL
7-9 years: 0.6-1.8 pg/mL
10-11 years: 0.1-3.5 pg/mL
12-13 years: 0.9-6.8 pg/mL
14-15 years: 1.2-7.5 pg/mL
16-17 years: 1.2-9.9 pg/mL
18-30 years: 0.8-7.4 pg/mL
31-40 years: 1.3-9.2 pg/mL
41-51 years: 1.1-5.8 pg/mL
Postmenopausal: 0.6-3.8 pg/mL
Tanner Stage I: Less than 2.2 pg/mL
Tanner Stage II: 0.4-4.5 pg/mL
Tanner Stage III: 1.3-7.5 pg mL
Tanner Stage IV: 1.1-15.5 pg/mL
Tanner Stage V: 0.8-9.2 pg/mL
1-6 years: Less than 0.6 pg/mL
7-9 years: 0.1-0.9 pg/mL
10-11 years: 0.1-6.3 pg/mL
12-13 years: 0.5-98.0 pg/mL
14-15 years: 3-138.0 pg/mL
16-17 years: 38.0-173.0 pg/mL
18 years and older: 47-244 pg/mL
Tanner Stage I: Less than or equal to 3.7 pg/mL
Tanner Stage II: 0.3-21 pg/mL
Tanner Stage III: 1.0-98.0 pg mL
Tanner Stage IV: 35.0-169.0 pg/mL
Tanner Stage V: 41.0-239.0 pg/mL

0081058Testosterone, Females or ChildrenEffective August 19, 2013
Premature (26-28 weeks)5-16 ng/dL59-125 ng/dL
Premature (31-35 weeks)5-22 ng/dL37-198 ng/dL
Newborn20-64 ng/dL75-400 ng/dL
1-5 monthsLess than 20 ng/dL14-363 ng/dL
6-24 monthsLess than 9 ng/dLLess than 37 ng/dL
2-3 yearsLess than 20 ng/dLLess than 15 ng/dL
4-5 yearsLess than 30 ng/dLLess than 19 ng/dL
6-7 yearsLess than 7 ng/dLLess than 13 ng/dL
8-9 years1-11ng/dL2-8 ng/dL
10-11 years3-32 ng/dL2-165 ng/dL
12-13 years6-50 ng/dL3-619 ng/dL
14-15 years6-52 ng/dL31-733 ng/dL
16-17 years9-58 ng/dL158-826 ng/dL
18-39 years9-55 ng/dL300-1080 ng/dL
40-59 years9-55 ng/dL300-890 ng/dL
60 years and older5-32 ng/dL300-720 ng/dL
Premenopausal (18 years and older)9-55 ng/dLDoes Not Apply
Postmenopausal5-32 ng/dLDoes Not Apply
Tanner Stage I2-17 ng/dL2-15 ng/dL
Tanner Stage II5-40 ng/dL3-303 ng/dL
Tanner Stage III10-63 ng/dL10-851 ng/dL
Tanner Stage IV-V11-62 ng/dL162-847 ng/dL

Interpretive Data
The concentrations of free and bioavailable testosterone are derived from mathematical expressions based on constants for the binding of testosterone to albumin and/or sex hormone binding globulin.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
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CPT Code(s)
Component Test Code*Component Chart NameLOINC
0081058Testosterone, LC-MS/MS2986-8
0081096Testosterone, Free LC-MS/MS2991-8
0081098Testosterone LC-MS, Bioavailable2990-0
0099375Sex Hormone Binding Globulin13967-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.