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Alpha Fetoprotein (Amniotic Fluid) with Reflex to Acetylcholinesterase and Fetal Hemoglobin
0080427
Ordering Recommendation

Evaluate possibility of a fetal open neural tube defect at 13-36 weeks of gestation.

Mnemonic
AFP AF
Methodology
Quantitative Chemiluminescent Immunoassay/Electrophoresis
Performed
Sun-Sat
Reported
3-4 days
Reflex: 3-11 days
New York DOH Approval Status
This test is New York DOH approved.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Amniocentesis. 
Collect
Amniotic fluid. 
Specimen Preparation
Transport 2.5 mL amniotic fluid. (Min: 1.5mL) 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Specimens contaminated with fetal blood. 
Remarks
Include gestational age at time of collection or estimated due date, physician name and phone number on the test request form. 
Stability
Ambient: 1 month; Refrigerated: 3 months; Frozen: 3 months 
Reference Interval
Test Number
Components
Reference Interval
 AFP, Amniotic FluidBy report
Ranges are based upon the weeks of gestation.
2006848Acetylcholinesterase and Fetal Hemoglobin, Amniotic FluidAcetylcholinesterase: Negative
Fetal Hemoglobin: Negative

Interpretive Data


Note
Information must include weeks of gestation. If the AFP (amniotic fluid) is elevated, then Acetylcholinesterase will be added. Additional charges apply. Acetylcholinesterase testing requires an additional 3-11 days to be reported.
CPT Code(s)
82106; if reflexed, add 82013 and 83033
Components
Component Test Code*Component Chart NameLOINC
0080015AFP, Amniotic Fluid1832-5
0080916Gestational Age (Complete)18185-9
0080929AFP AF Interpretation41273-4
0080934Multiple of Median29595-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Fetoprotein, Amniotic Fluid
  • AFP
  • AFP-AF (Alpha-Fetoprotein, Amniotic Fluid)
  • Alpha Fetoprotein, Amniotic Fluid
  • Alpha-Fetoprotein, Amniotic Fluid