5-Hydroxyindoleacetic Acid (HIAA), Urine
0080420
Ordering Recommendation
Submit With Order
Mnemonic
HIAA
Methodology
Quantitative High Performance Liquid Chromatography
Performed
Sun, Tue-Sat
Reported
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Patients should abstain, if possible, from medications, over-the-counter drugs, and herbal remedies for at least 72 hours prior to the test. Foods rich in serotonin (avocados, bananas, eggplant, pineapple, plums, tomatoes, walnuts) and medications that may affect metabolism of serotonin must be avoided at least 72 hours before and during collection of urine for HIAA.  
Collect
24-hour or random urine. Refrigerate 24-hour specimens during collection.  
Specimen Preparation
Transfer 4 mL aliquot from a well-mixed 24-hour or random collection to an ARUP Standard Transport Tube. (Min: 1 mL) Record total volume and collection time interval on transport tube and test request form.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Any sample except urine.  
Remarks
Please see Note for a more comprehensive list of dietary restrictions.  
Stability
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 2 weeks  
Reference Interval
Test Number Components Reference Interval
5-​HIAA, Urine0.0-​15.0 mg/d
5-​HIAA, Urine The HIAA-​to-​creatinine ratio will be reported whenever the urine collection is random or other than 24 hours, or the urine volume is less than 400 mL/24 hours.

0-​14 mg/g crt
Creatinine, Urine -​ per 24h
Age Male Female
3-​8 years 140-​700 mg/d 140-​700 mg/d
9-​12 years 300-​1300 mg/d 300-​1300 mg/d
13-​17 years 500-​2300 mg/d 400-​1600 mg/d
18-​50 years 1000-​2500 mg/d 700-​1600 mg/d
51-​80 years 800-​2100 mg/d 500-​1400 mg/d
81 years and older 600-​2000 mg/d 400-​1300 mg/d
Interpretive Data
5-Hydroxyindoleacetic acid (5-HIAA) results are expressed as a ratio to creatinine excretion (mg/g CRT). HIAA mass per day (mg/d) is not reported if the urine collection is random, other than 24 hours, or for a urine volume less than 400 mL/d. No reference interval is available for results reported in units of mg/L.
Note
Foods and medications associated with altered urinary HIAA results:

Decreased HIAA
: Aspirin, chlorpromazine (Thorazine), corticotropin, dihydroxyphenylacetic acid, alcohol, gentisic acid, homogentisic acid, hydrazine derivatives, imipramine (Tofranil®), isocarboxazid (Marplan), keto acids, levodopa, MAO inhibitors, methenamine, methyldopa (Aldomet®), perchlorperazine, phenothiazines (Compazine®), promazine, promethazine (Mepergan®).

Increased HIAA:
Acetaminophen, acetanilide, caffeine, coumaric acid, diazepam (Valium®), ephedrine, fluorouracil, glycerol guaiacolate (Guaifenesin), melphalan (Alkeran®), mephenesin, methamphetamine (Desoxyn), methocarbamol (Robaxin®), naproxen, nicotine, phenacetin, phenmetrazine, phenobarbital, phentolamine, rauwolfia, reserpine.

CPT Code(s)
83497
Components
Component Test Code*Component Chart Name
0020207Creatinine, Urine - per volume
0020208Creatinine, Urine - per 24h
00802385-HIAA Urine Interpretation
00803205-HIAA Urine - per 24h
00803215-HIAA Urine - per volume
00813435-HIAA Urine - ratio to CRT
0097110Total Volume
0097111Hours Collected
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • 5-HIAA
  • 5-OH-Indoleacetic Acid
  • Hydroxyindoleacetic Acid
  • Serotonin Metabolite