Vitamin D, 1, 25-Dihydroxy
Ordering Recommendation

May be useful for evaluating calcium metabolism in individuals with hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing. Test is not appropriate for diagnosing vitamin D deficiency or insufficiency.

VIT D 1,25
Quantitative Chemiluminescent Immunoassay
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube or plain red, lithium heparin or EDTA plasma. 
Specimen Preparation
Allow serum separator or plain red tube to sit for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)
Storage/Transport Temperature: Refrigerated. 
Storage/Transport Temperature
Unacceptable Conditions
Grossly hemolyzed or lipemic specimens. 
After separation from cells: Ambient: 1 week; Refrigerated: 2 weeks; Frozen: 6 months 
Reference Interval
19.9-79.3 pg/mL
Interpretive Data
This test is primarily indicated during patient evaluation for hypercalcemia and renal failure. A normal result does not rule out Vitamin D deficiency. The recommended test for diagnosing Vitamin D deficiency is Vitamin D 25-hydroxy.

Component Test Code*Component Chart NameLOINC
0080385Vitamin D, 1,25-Dihydroxy1649-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 1,25-(OH)2-D
  • 1,25-Dihydroxy Vitamin D
  • 1,25-Dihydroxycholecalciferol
  • 1,25-Dihydroxyvitamin D
  • 1,25-Dihydroxyvitamin D3
  • Calcitriol
  • D, 1-25 Dihydroxy, Vitamin
  • Vit D 1,25