Vitamin D, 1, 25-Dihydroxy
0080385
Ordering Recommendation
Preferred test for individuals with hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing. Test is not appropriate for diagnosing vitamin D insufficiency.
Submit With Order
Mnemonic
VIT D 1,25
Methodology
Quantitative Radioimmunoassay
Performed
Sun-Sat
Reported
2-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube or plain red. Also acceptable: Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Allow serum separator or plain red tube to sit for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
 
Remarks
 
Stability
After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
15-75 pg/mL
Interpretive Data
This test is primarily indicated during patient evaluation for hypercalcemia and renal failure. A normal result does not rule out Vitamin D deficiency. The recommended test for diagnosing Vitamin D deficiency is Vitamin D 25-hydroxy.

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
82652
Components
Component Test Code*Component Chart Name
0080385Vitamin D, 1,25-Dihydroxy
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • 1,25-(OH)2-D
  • 1,25-Dihydroxy Vitamin D
  • 1,25-Dihydroxycholecalciferol
  • 1,25-Dihydroxyvitamin D
  • 1,25-Dihydroxyvitamin D3
  • Calcitriol
  • D, 1-25 Dihydroxy, Vitamin
  • Vit D 1,25