Vitamin D, 1, 25-Dihydroxy
Ordering Recommendation
Preferred test for individuals with hypercalcemia or renal failure in addition to Vitamin D, 25-Hydroxy testing. Test is not appropriate for diagnosing vitamin D insufficiency.
VIT D 1,25
Quantitative Radioimmunoassay
2-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube or plain red. Also acceptable: Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Allow serum separator or plain red tube to sit for 15-20 minutes at room temperature for proper clot formation. Centrifuge and separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
15-75 pg/mL  
Interpretive Data
This test is primarily indicated during patient evaluation for hypercalcemia and renal failure. A normal result does not rule out Vitamin D deficiency. The recommended test for diagnosing Vitamin D deficiency is Vitamin D 25-hydroxy.

See Compliance Statement B:
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0080385Vitamin D, 1,25-Dihydroxy1649-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • 1,25-(OH)2-D
  • 1,25-Dihydroxy Vitamin D
  • 1,25-Dihydroxycholecalciferol
  • 1,25-Dihydroxyvitamin D
  • 1,25-Dihydroxyvitamin D3
  • Calcitriol
  • D, 1-25 Dihydroxy, Vitamin
  • Vit D 1,25