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Vitamin D, 25-Hydroxy
0080379
Ordering Recommendation

Preferred test to diagnose vitamin D insufficiency and monitor response to therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.

Mnemonic
VIT D 25
Methodology
Quantitative Chemiluminescent Immunoassay
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Transfer 1 mL serum  to an ARUP Standard Transport Tube. (Min: 0.3 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
EDTA plasma, tissue or urine. Grossly hemolyzed or lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months 
Reference Interval
0-17 years
DeficiencyLess than 20 ng/mL
Optimum levelGreater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

18 years and older
DeficiencyLess than 20 ng/mL
Insufficiency20-29 ng/mL
Optimum Level30-80 ng/mL
Possible ToxicityGreater than 150 ng/mL

Interpretive Data
This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy.

Note
CPT Code(s)
82306
Components
Component Test Code*Component Chart NameLOINC
0080379Vitamin D 25 OH1989-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • D, 25-Hydroxy, Vitamin
  • Ergocalciferol
  • Vit D
  • vitamin D