Vitamin D, 25-Hydroxy
0080379
Ordering Recommendation
Use to diagnose vitamin D insufficiency and monitor response to vitamin D therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.
Submit With Order
Mnemonic
VIT D 25
Methodology
Quantitative Chemiluminescent Immunoassay
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
EDTA plasma, tissue or urine. Grossly hemolyzed or lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
0-​17 years
Deficiency Less than 20 ng/mL
Optimum level Greater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-​52.)

18 years and older
Deficiency Less than 20 ng/mL
Insufficiency 20-​29 ng/mL
Optimum Level 30-​80 ng/mL
Possible Toxicity Greater than 150 ng/mL
Interpretive Data
This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy.
Note
CPT Code(s)
82306
Components
Component Test Code*Component Chart Name
0080379Vitamin D 25 OH
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • D, 25-Hydroxy, Vitamin
  • Ergocalciferol
  • Vit D
  • vitamin D