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Vitamin D, 25-Hydroxy
0080379
Ordering Recommendation
Use to diagnose vitamin D insufficiency and monitor response to vitamin D therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.
Mnemonic
VIT D 25
Methodology
Quantitative Chemiluminescent Immunoassay
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Transfer 1 mL serum  to an ARUP Standard Transport Tube. (Min: 0.3 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
EDTA plasma, tissue or urine. Grossly hemolyzed or lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 months  
Reference Interval
0-17 years
DeficiencyLess than 20 ng/mL
Optimum levelGreater than or equal to 20 ng/mL*
*(Wagner CL et al. Pediatrics 2008; 122: 1142-52.)

18 years and older
DeficiencyLess than 20 ng/mL
Insufficiency20-29 ng/mL
Optimum Level30-80 ng/mL
Possible ToxicityGreater than 150 ng/mL

Interpretive Data
This assay accurately quantifies the sum of vitamin D3, 25-Hydroxy and vitamin D2, 25-Hydroxy.


Note
CPT Code(s)
82306
Components
Component Test Code*Component Chart NameLOINC
0080379Vitamin D 25 OH1989-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • D, 25-Hydroxy, Vitamin
  • Ergocalciferol
  • Vit D
  • vitamin D