Maternal Serum Screen, Alpha Fetoprotein, hCG, Estriol, and Inhibin A
Ordering Recommendation
Second trimester screening test for DS, T18, and ONTD.
Quantitative Chemiluminescent Immunoassay
2-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Specimen must be drawn between 14 weeks, 0 days and 24 weeks, 6 days gestation.  
Serum separator tube or plain red.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 3 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Specimens exposed to repeated freeze/thaw cycles. Hemolyzed specimens.  
The following information is required and must accompany the sample for test interpretation: patient's date of birth, current weight, due date, dating method (US, LMP), number of fetuses present, patient's race, if the patient requires insulin, if there is a known family history of neural tube defects, if the patient has had a previous pregnancy with a chromosome abnormality, if the patient is taking valproic acid or carbamazepine (Tegretol®), physician's name and phone number; and for in vitro fertilization pregnancies, the age of the egg donor.  
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 1 year  
Reference Interval
By report

Includes AFP, hCG, Estriol, and Inhibin A.
Intervals are based upon weeks of gestation.  
Interpretive Data
This test is used to screen for fetal risk of Down syndrome (trisomy 21), trisomy 18, and Open Neural Tube Defect (ONTD, spina bifida).
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0080021Patient's AFP1834-1
0080241Estimated Due Date11778-8
0080267MoM for DIA35738-4
0080268Patient's DIA23883-2
0080917Maternal Weight29463-7
0080918MoM for AFP20450-3
0080920Maternal Screen Interpretation49586-1
0080924Insulin Req Maternal Diabetes44877-9
0080925Family Hx Neural Tube Defect8670-2
0080926Maternal Race21484-1
0080927Number of Fetuses11878-6
0080932Maternal Age At Delivery21612-7
0080935Patient's hCG19080-1
0080937MoM For hCG20465-1
0080938Gestational Age (Exact)18185-9
0080941Patient's uE32250-9
0080943MoM for uE320466-9
0081158Family History of Aneuploidy32435-0
2002850EER Maternal Screen AFP, hCG, EST, INH11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • AFP 4 Marker Screen
  • AFP MS4 (Quad)
  • Alpha Fetoprotein, hCG, Estriol, and Inhibin
  • Maternal Screening, AFP 4 Marker
  • QUAD
  • Quad AFP
  • Quad screening