Vitamin B6 (Pyridoxal 5-Phosphate)
Ordering Recommendation
Quantitative High Performance Liquid Chromatography
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Collect specimen after an overnight fast.  
Green (sodium or lithium heparin) or plasma separator tube. Also acceptable: Plain red.  
Specimen Preparation
Protect from light during collection, storage, and shipment. Separate plasma or serum from cells within 1 hour of collection. Transfer 1 mL plasma or serum to an ARUP Amber Transport Tube and freeze immediately. (Min: 0.5 mL)  
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.  
Unacceptable Conditions
Any specimens other than heparinized plasma or serum. Non-frozen specimens. Serum separator tubes or EDTA preserved tubes. Hemolyzed specimens.  
After separation from cells: Ambient: Unacceptable; Refrigerated: 4 hours; Frozen: 2 months  
Reference Interval
20-125 nmol/L  
Interpretive Data
Pyridoxal 5'-phosphate measured in a specimen collected following an 8 hour or overnight fast accurately indicates vitamin B6 nutritional status. Non-fasting specimen concentration reflects recent vitamin intake.

See Compliance Statement B:
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
This test measures pyridoxal 5-phosphate, the biologically active form of vitamin B6.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0080111Vitamin B6 (Pyridoxal 5-Phosphate)2900-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • P 5-P
  • Pyridoxal Phosphate