Maternal Serum Screen, Alpha Fetoprotein, hCG, and Estriol
Ordering Recommendation
Quantitative Chemiluminescent Immunoassay
2-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Specimen must be drawn between 14 weeks, 0 days and 24 weeks, 6 days gestation.  
Serum separator tube or plain red.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Specimens exposed to repeated freeze/thaw cycles. Hemolyzed specimens.  
The following information is required and must accompany the sample in order for testing to be interpreted: patient's date of birth, current weight, due date, dating method (US, LMP), number of fetuses present, patient's race, if the patient requires insulin, if there is a known family history of neural tube defects, if the patient has had a previous pregnancy with a chromosome abnormality, if the patient is taking valproic acid or carbamazepine (Tegretol®), physician's name and phone number; and for in vitro fertilization pregnancies, the age of the egg donor.  
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 1 year  
Reference Interval
By report

Includes AFP, hCG, and Estriol.
Intervals are based upon weeks of gestation.  
Interpretive Data
This test is used to screen for fetal risk of Down syndrome (trisomy 21), trisomy 18, and Open Neural Tube Defect (ONTD, spina bifida).
CPT Code(s)
(82105; 84702; 82677) or 81510*

*The 2014 AMA CPT manual contains the component CPT Codes and the new MAAA codes. Please direct any questions regarding CPT coding to the payer being billed.
Component Test Code*Component Chart Name
0080021Patient's AFP
0080241Estimated Due Date
0080917Maternal Weight
0080918MoM for AFP
0080920Maternal Screen Interpretation
0080924Insulin Req Maternal Diabetes
0080925Family Hx Neural Tube Defect
0080926Maternal Race
0080927Number of Fetuses
0080932Maternal Age At Delivery
0080935Patient's hCG
0080937MoM For hCG
0080938Gestational Age (Exact)
0080941Patient's uE3
0080943MoM for uE3
0081158Family History of Aneuploidy
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Alpha Fetoprotein, hCG, and Estriol
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