Proinsulin, Intact/Insulin Ratio
Ordering Recommendation

Aids in the detection of insulinoma. Do not use to diagnose diabetes mellitus.

Quantitative Chemiluminescent Immunoassay/Quantitative Chemiluminescent Immunoassay
Refer to individual components
1-6 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Patient must be fasting for 12-15 hours prior to collection. 
Serum separator tube. Also acceptable: Lavender (EDTA) or pink (K2EDTA). 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube and freeze immediately. (Min: 0.8 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Heparinized plasma. Vitreous or I.V. fluids. Hemolyzed specimens. 
After separation from cells: Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 2 months (avoid repeated freeze/thaw cycles) 
Reference Interval
Available Separately
Reference Interval
Yes (0070063)Insulin, Fasting3-19 µIU/mL
Yes (0070112)Proinsulin, Intact0-17 years: Not established
Effective May 19th, 2014
18 years and older: Less than or equal to 8.0 pmol/L
NoProinsulin, Intact/Insulin Ratio CalculationProinsulin, Intact/Insulin Ratio as Percent:
0-17 years: Not established
18 years and older: 0.8-21.7 percent

Interpretive Data
Insulin, Fasting: This test reacts on a nearly equimolar basis with the analogs insulin aspart, insulin glargine, and insulin lispro. Insulin detemir exhibits approximately 50 percent cross-reactivity. Test reactivity with insulin glulisine is negligible (less than 3 percent). To convert to pmol/L, multiply µIU/mL by 6.0.

Proinsulin, Intact: Fasting intact proinsulin values above the reference interval indicate a possible insulin secreting pancreatic tumor (insulinoma) in patients with hypoglycemia. Fasting intact proinsulin values range from 3 to 50 pmol/L in patients with untreated type 2 diabetes.

Component Test Code*Component Chart NameLOINC
0070063Insulin, Fasting27873-9
0070112Proinsulin, Intact10451-3
0070257Proinsulin, Intact/Insulin Ratio Calc
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.