Insulin, Free and Total
0070155
Ordering Recommendation
Not recommended to diagnose diabetes mellitus.
Mnemonic
INS F&T
Methodology
Quantitative Ultrafiltration/Quantitative Chemiluminescent Immunoassay
Performed
Mon, Wed-Sat
Reported
2-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube or plain red. Also acceptable: Lavender (EDTA), or pink (K2EDTA).  
Specimen Preparation
Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.1 mL)  
Storage/Transport Temperature
Frozen. Separate specimens must be submitted when multiple tests are ordered.  
Unacceptable Conditions
Heparinized specimens. Sodium fluoride/potassium oxalate plasma. Hemolyzed specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month  
Reference Interval
Effective August 19, 2013
 
Test Number Components Reference Interval
 Insulin, Free 3-​19 µIU/mL
 Insulin, Total 3-​19 µIU/mL
Interpretive Data
This test reacts on a nearly equimolar basis with the analogs insulin aspart, insulin glargine, and insulin lispro. Insulin detemir exhibits approximately 50 percent cross-reactivity. Test reactivity with insulin glulisine is negligible (<3 percent). To convert to pmol/L, multiply µIU/mL by 6.0.

Reference intervals established for fasting specimens.  
Note
 
CPT Code(s)
83525; 83527
Components
Component Test Code*Component Chart Name
0070091Insulin Free
0070092Total Insulin
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Free and Total insulin