Insulin, Free and Total
0070155
Ordering Recommendation
Not recommended to diagnose diabetes mellitus.
Mnemonic
INS F&T
Methodology
Quantitative Ultrafiltration/Quantitative Chemiluminescent Immunoassay
Performed
Mon, Wed-Sat
Reported
2-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Fasting specimen preferred.  
Collect
Serum separator tube or plain red. Also acceptable: Lavender (EDTA), or pink (K2EDTA).  
Specimen Preparation
Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1.1 mL)  
Storage/Transport Temperature
Frozen. Separate specimens must be submitted when multiple tests are ordered.  
Unacceptable Conditions
Heparinized specimens. Sodium fluoride/potassium oxalate plasma. Hemolyzed specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month  
Reference Interval
Effective August 19, 2013
 
Test Number Components Reference Interval
 Insulin, Free 3-​19 µIU/mL
 Insulin, Total 3-​19 µIU/mL
Interpretive Data
This test reacts on a nearly equimolar basis with the analogs insulin aspart, insulin glargine, and insulin lispro. Insulin detemir exhibits approximately 50 percent cross-reactivity. Test reactivity with insulin glulisine is negligible (<3 percent). To convert to pmol/L, multiply µIU/mL by 6.0.

Reference intervals established for fasting specimens.  
Note
 
CPT Code(s)
83525; 83527
Components
Component Test Code*Component Chart Name
0070091Insulin Free
0070092Total Insulin
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Free and Total insulin