Cyclosporine A by Tandem Mass Spectrometry
0070035
Ordering Recommendation
 
Mnemonic
CYA
Methodology
Quantitative Liquid Chromatography-Tandem Mass Spectrometry
Performed
Sun-Sat
Reported
Within 24 hours  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Pre-dose (trough) levels should be drawn.  
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transfer 1 mL well-mixed whole blood to a plastic vial. (Min: 0.25 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Serum or plasma. Specimens left at room temperature for longer than 24 hours. Clotted specimens.  
Remarks
  
Stability
Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 2 months  
Reference Interval
Effective February 18, 2014
 
 Therapeutic Range:
Kidney transplant (in combination with Everolimus): 1 month post-​transplant: 100-​200 ng/mL
2-​3 months post-​transplant: 75-​150 ng/mL
4-​5 months post-​transplant: 50-​100 ng/mL
6-​12 months post-​transplant: 25-​50 ng/mL
Heart transplant: Up to 3 months post-​transplant: 350-​525 ng/mL
4 months and older post-​transplant: 145-​350 ng/mL
Liver transplant: 290-​525 ng/mL
Toxic value: Greater than 700 ng/mL
Interpretive Data
The optimal therapeutic range for a given patient may differ from this suggested range based on the indication for therapy, treatment phase (initiation or maintenance), use in combination with other drugs, time of specimen collection relative to prior dose, type of transplanted organ, and/or the therapeutic approach of the transplant center.

See Compliance Statement B: www.aruplab.com/CS
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
Cyclosporine (Sandimmune) whole blood concentrations can be measured by either chromatographic or immunoassay methodologies. These two methodologies are not directly interchangeable, and the measured cyclosporine whole blood concentration depends on the methodology used. Reference ranges may vary according to the specific immunoassay or HPLC-MS/MS test. Generally, immunoassays have been reported to have a positive bias relative to HPLC-MS/MS assays due to the detection of antibody cross-reactivity with cyclosporine metabolites.
CPT Code(s)
80158
Components
Component Test Code*Component Chart Name
0070035Cyclosporine A by HPLC-MS/MS
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Cyclosporine
  • Gengraf
  • Neoral
  • Restasis
  • Sandimmune