Acid-Fast Bacillus (AFB) Identification
Ordering Recommendation

Order for M. tuberculosis complex speciation.

Identification. Methods may include biochemical, mass spectrometry, nucleic acid probe, polymerase chain reaction, or sequencing.
Varies (depends on organism growth)
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Actively growing organism, in pure culture. 
Specimen Preparation
Place each specimen in an individually sealed bag. Transport sealed container with pure culture on solid or in liquid media. 
Storage/Transport Temperature
Room temperature. If culture is suspected of being a microorganism listed as infectious substance affecting humans on IATA list, submit specimen according to Biological Substance, Category A, shipping guidelines. 
Unacceptable Conditions
Mixed cultures or non-viable organisms. Organisms submitted on an agar plate. 
Indicate source of organism and any other pertinent information, such as the growth rate of the organism or DNA probe results. Probes can be performed directly from Middlebrook 7H9 broth. MGIT is not suitable for direct probe testing. 
Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks 
Reference Interval
Complete identification of clinically significant isolates.
Interpretive Data
For identification by DNA sequencing and MALDI-TOF:

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

A processing fee will be billed for all organisms submitted that are not in pure culture, as indicated in specimen requirements.
Identification of AFB isolates is billed by individual tests performed.
CPT Code(s)
CPT codes for identification vary based on method.
Component Test Code*Component Chart NameLOINC
0060999AFB Identification543-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • AFB identification by sequencing, 16S rRNA
  • M. tuberculosis identification
  • Mycobacterium tuberculosis Probe