Acid-Fast Bacillus (AFB) Identification
0060999
 
Ordering Recommendation
Mnemonic
MC AFBID
Methodology
Identification. Methods may include biochemical, mass spectrometry, nucleic acid probe, polymerase chain reaction, or sequencing.
Performed
Sun-Sat
Reported
Varies (depends on organism growth)
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Actively growing organism, in pure culture.  
Specimen Preparation
Transport sealed container with pure culture on solid or in liquid media. Place each specimen in an individually sealed bag.  
Storage/Transport Temperature
Room temperature. Submit specimen according to Infectious Substance, Category A, shipping guidelines.

Submit specimen according to Infectious Substance, Category A, shipping guidelines.  
Unacceptable Conditions
Mixed cultures or non-viable organisms. Organisms submitted on an agar plate.  
Remarks
Indicate source of organism and any other pertinent information, such as the growth rate of the organism or DNA probe results. Probes can be performed directly from Middlebrook 7H9 broth. MGIT is not suitable for direct probe testing.  
Stability
Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks  
Reference Interval
Complete identification of clinically significant isolates.
Interpretive Data
For identification by DNA sequencing:

See Compliance Statement B: www.aruplab.com/CS
Note
A processing fee will be billed for all organisms submitted that are not in pure culture, as indicated in specimen requirements.
Identification of AFB isolates is billed by individual probes and tests performed.
CPT Code(s)
CPT codes for identification vary based on method.
Components
Component Test Code*Component Chart Name
0060999AFB Identification
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • AFB identification by sequencing, 16S rRNA
  • AFBSEQ
  • M. tuberculosis identification
  • MTBGP
  • Mycobacterium tuberculosis Probe