Chlamydia trachomatis DFA
Ordering Recommendation
Not recommended for detecting Chlamydia trachomatis. Preferred test is one of the nucleic acid amplification tests (NAAT) [ie, Chlamydia trachomatis by Transcription-Mediated Amplification (TMA) (0060243) or Chlamydia trachomatis and Neisseria gonorrhoeae by Transcription-Mediated Amplification (TMA) (0060241)].
Direct Fluorescent Antibody Stain
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Eye, genital, nasopharyngeal, or rectal swab.
For Chlamydia DFA only, collection kits with fixative are available (ARUP supply #16578). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Specimen Preparation
Fix slide as directed by collection kit. Transport fixed slide.  
Storage/Transport Temperature
Room temperature.  
Unacceptable Conditions
Swabs in gel. Transport systems designed for use with other methodologies of Chlamydia testing. Dry swabs.  
Specimen source preferred.  
Fixed Slide: Ambient: 1 week; Refrigerated: 1 week; Frozen: 1 week  
Reference Interval
Interpretive Data
Sensitivity of this method is dependent on adequacy of the smear. If there are fewer than 20 columnar epithelial cells, the result will be reported as "sample inadequate."

Amplified DNA testing is recommended for detection of Chlamydia trachomatis from endocervical or urethral specimens. Refer to Chlamydia trachomatis by Amplified Detection (APTIMA®) (0060243). Specimen must be collected and transported with test-specific kit. Culture is recommended for Chlamydia trachomatis detection in suspected sexual abuse and for suspected failure of therapy. Refer to Chlamydia trachomatis Culture (Genital) (0060850).
CPT Code(s)
Component Test Code*Component Chart Name
0060287Chlamydia trachomatis DFA
0060338Chlamydia trachomatis DFA Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at
Cross References
  • C trachomatis DFA
  • Chlamydia trachomatis, Fluorescence Staining of MicroTrak Slides