Varicella-Zoster Virus and Herpes Simplex Virus DFA with Reflex to Varicella-Zoster Virus Culture and Herpes Simplex Virus Culture
Ordering Recommendation
Submit With Order
Direct Fluorescent Antibody Stain/Cell Culture
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Collect vesicle specimen during first 3 days of rash.  
Vesicle fluid or swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.  
Specimen Preparation
Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Unacceptable Conditions
Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.  
Specimen source preferred.  
Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable  
Reference Interval
Interpretive Data
If DFA results are negative or inadequate, then VZV and HSV cultures will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If the specimen contains fewer than 20 cells, then DFA results will be reported as "inadequate."
CPT Code(s)
87290; 87274; 87273; if reflexed, add 87252 x2; 87254; 87253
Component Test Code*Component Chart Name
0060302Varicella-Zoster Virus, DFA with Reflex
0060303Herpes Simplex Virus 1, DFA
0060304Herpes Simplex Virus 2, DFA
0060336VZV HSVFAC Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Herpes Culture
  • Herpes Simplex Viral Culture
  • Herpes Simplex Virus Antigen Detection, DFA.
  • Herpes Zoster
  • Herpes Zoster Antigen Detection & Culture
  • Herpes Zoster Antigen Detection and Culture
  • HSV 1 and HSV 2
  • Varicella Zoster Virus
  • Varicella Zoster Virus Antigen Detection
  • VZV
  • VZV Antigen Detection and Culture