Detect varicella-zoster virus (VZV). Negative or inadequate DFA results are confirmed with culture. DFA sensitivity is highest when performed on scrapings from base of lesions; culture sensitivity is highest when specimens are collected soon after onset of symptoms. Molecular testing is generally preferred; refer to VZV by PCR (0060042).
DFA: Within 24 hours; Culture: 3-5 days
- Patient Preparation
- Collect vesicle specimen during first three days of rash.
- Vesicle fluid or swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.
- Specimen Preparation
- Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive). Available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787.
- Storage/Transport Temperature
- Unacceptable Conditions
- Bone marrow CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.
- Specimen source preferred.
- Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable
Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."
|Component Test Code*||Component Chart Name||LOINC|
|0060282||Varicella-Zoster Virus, DFA||5882-6|
|0060335||Varicella-Zoster Virus Fac Source|
- Herpes Zoster
- Herpes Zoster Antigen Detection & Culture
- Varicella Zoster Virus
- Varicella Zoster Virus Antigen Detection
- VZV DFA