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Varicella-Zoster Virus DFA with Reflex to Varicella-Zoster Virus Culture
0060282
Ordering Recommendation

Detect varicella-zoster virus (VZV). Negative or inadequate DFA results are confirmed with culture. DFA sensitivity is highest when performed on scrapings from base of lesions; culture sensitivity is highest when specimens are collected soon after onset of symptoms. Molecular testing is generally preferred; refer to VZV by PCR (0060042).

Mnemonic
VZVFAC
Methodology
Direct Fluorescent Antibody Stain/Cell Culture
Performed
Sun-Sat
Reported
3-5 days
DFA: Within 24 hours; Culture: 3-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect vesicle specimen during first three days of rash.  
Collect
Vesicle fluid or swab, tissue, or skin scrapings. Swab should be rolled in base of fresh vesicle to obtain cells.  
Specimen Preparation
Immediately transfer specimen to viral transport media (ARUP Supply #12884) and refrigerate (extremely temperature sensitive). Available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Bone marrow CSF, or whole blood. Calcium alginate, eSwab, dry or wood swabs.  
Remarks
Specimen source preferred.  
Stability
Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: Unacceptable  
Reference Interval
Negative
Interpretive Data
Approximately 20% of samples submitted for VZV are positive for herpes simplex virus. Testing for herpes simplex virus is NOT included in this test.


Note
If DFA is negative or inadequate, then a VZV culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."
CPT Code(s)
87290; if reflexed, add 87252; 87254
Components
Component Test Code*Component Chart NameLOINC
0060282Varicella-Zoster Virus, DFA5882-6
0060335Varicella-Zoster Virus Fac Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Herpes Zoster
  • Herpes Zoster Antigen Detection & Culture
  • Varicella Zoster Virus
  • Varicella Zoster Virus Antigen Detection
  • VZV
  • VZV DFA