Respiratory Viruses DFA with Reflex to Viral Culture, Respiratory
0060281
Ordering Recommendation
Order this test when the detection of respiratory viruses in addition to influenza is important. Test detects influenza (A, B), parainfluenza (1, 2, 3), adenovirus, respiratory syncytial virus (RSV), and human metapneumovirus (hMPV). Molecular methods are preferred for immunocompromised and hospitalized patients. For general inpatient workup of influenza-like illness, order the Respiratory Virus Mini Panel by PCR (0060764).
Mnemonic
RSPFAC
Methodology
Direct Fluorescent Antibody Stain/Cell Culture
Performed
Sun-Sat
Reported
Within 24 hours  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Respiratory specimen: Bronchoalveolar lavage (BAL), nasopharyngeal (aspirate, swab, or washing), or tracheal aspirate.  
Specimen Preparation
Fluid: Transfer specimen to a sterile container. Also acceptable: Transfer to viral transport media (ARUP supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab
: Place in viral transport media.
Place each specimen in a separate, individually sealed bag.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Calcium alginate, dry or wood swabs. Slides.  
Remarks
Specimen source preferred.  
Stability
Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Unacceptable  
Reference Interval
Negative  
Interpretive Data
 
Note
If DFA is negative or inadequate, then a respiratory viral culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate." 
CPT Code(s)
87276; 87275; 87279 x3; 87280; 87260; 87299; if reflexed add 87252; if definitive identification performed, add 87253.
Components
Component Test Code*Component Chart NameLOINC
0060294Influenza A - DFA5863-6
0060295Influenza B - DFA5867-7
0060296Parainfluenza Type 1 - DFA5869-3
0060297Parainfluenza Type 2 - DFA5871-9
0060298Parainfluenza Type 3 - DFA5873-5
0060299Adenovirus - DFA5834-7
0060301Respiratory Syncytial Virus - DFA5877-6
0060334RSP Fac Source 
0060778Human Metapneumovirus DFA40979-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Adenovirus
  • CMV culture
  • Culture for virus
  • cytomegalovirus
  • enterovirus culture
  • herpes simplex virus
  • HMPV
  • HSV culture
  • human mentapneumovirus by DFA
  • Influenza A
  • influenza A and B culture
  • Influenza B, Parainfluenza Types 1, 2, 3,
  • parainfluenza types 1, 2, 3
  • Respiratory profile, direct detection DFA
  • Respiratory Virus Screen, DFA
  • RSV
  • RSV culture
  • varicella-zoster virus
  • VSV culture