Herpes Simplex Virus DFA with Reflex to Herpes Simplex Virus Culture
0060280
 
Ordering Recommendation
Alternate test for detection of herpes simplex virus infection. Negative DFA result reflexes to HSV culture for confirmation. Molecular testing is generally preferred.
Mnemonic
HSVFAC
Methodology
Direct Fluorescent Antibody Stain/Cell Culture
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Buccal mucosa lesion, cervical or genital lesion, eye, throat or vesicle swab, bronchoalveolar lavage (BAL), tissue or vesicle fluid.  
Specimen Preparation
Fluid: Transfer specimen to a sterile container. Also acceptable: Transfer to viral transport media (ARUP Supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Swab or Tissue:
Place in viral transport media. Place each specimen in an individually sealed bag.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Bone marrow, CSF, or whole blood. Calcium alginate, eSwab, dry, or wood swabs.  
Remarks
Specimen source preferred.  
Stability
Ambient: 4 hours; Refrigerated: 72 hours; Frozen: Unacceptable  
Reference Interval
Negative
Interpretive Data
Note
If DFA is negative or inadequate, then a HSV culture will be added. Additional charges apply.

Sensitivity of DFA methodology is dependent upon adequacy of the specimen. If specimen contains fewer than 20 cells, then the DFA result will be reported as "inadequate."
CPT Code(s)
87274; 87273; if reflexed, add 87252; 87253.
Components
Component Test Code*Component Chart Name
0060292Herpes Simplex Virus 1, DFA
0060293Herpes Simplex Virus 2, DFA
0060332HSVFAC Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Herpes Culture
  • Herpes Simplex Viral Culture
  • Herpes Simplex Virus Antigen Detection, DFA
  • Herpes Simplex Virus by DFA
  • HHV1
  • HHV2
  • HSV DFA
  • HSV-1
  • HSV-2