Antimicrobial Susceptibility - Bactericidal Assay
Ordering Recommendation
Broth Microdilution
3-6 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Actively growing isolate in pure culture AND serum or body fluid. Collect using aseptic technique.  
Specimen Preparation
Organism: Transport sealed container with pure culture on agar slant or in bacterial transport media. Place each specimen in an individually sealed bag.
AND Serum or Body Fluid:
Transfer 2 mL serum or body fluid to a sterile container and freeze. (Min: 1 mL)  
Storage/Transport Temperature
Organism: Room temperature.
Serum or Body Fluid:
Unacceptable Conditions
Mixed cultures or non-viable organisms.  
Isolate identification and specimen source required. A list of all antibiotics the patient is receiving or has received in the past 48 hours must be provided (this information is essential for test performance; missing information may cause delays in turnaround time).  
Organism: Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable
Serum or Body Fluid:
Ambient: 2 hours; Refrigerated: 24 hours; Frozen: 1 week  
Reference Interval
Interpretive report provided.
Interpretive Data
The data supporting the use of bactericidal assays in the management of complicated bacterial infections, such as endocarditis and osteomyelitis, are limited. Results should be interpreted with caution.
If more than one antibiotic is being used, the peak sample should be drawn after completion of the second antibiotic. Both peak and trough levels are usually obtained for bactericidal assays. Results may be useful in following efficacy of treatment, particularly in patients with complicated bacterial infections.

An additional processing fee will be billed for all organisms not submitted in pure culture, as indicated in the specimen requirements.

If species identification is not provided, identification will be performed at ARUP. Additional charges apply.
CPT Code(s)
Component Test Code*Component Chart Name
0060204Antimicrobial - Bactericidal Assay
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at
Cross References
  • BCT
  • Schlichter Assay
  • Schlichter Test
  • Serum Bactericidal Assay