Leptospira Culture
0060158
 
Time SensitiveTime Sensitive
Ordering Recommendation
Mnemonic
MC LEPTO
Methodology
Culture
Performed
Sun-Sat
Reported
Within 42 days
Positives are reported as soon as detected.
Final: Negative at 6 weeks
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
CSF or blood should be collected within first week of illness; urine after the first week of illness. Blood is collected per blood culture procedure.  
Collect
Green (sodium heparin) or sodium oxalate. Also acceptable: Bactec aerobic resin bottle or other commercial blood culture bottles, CSF, or urine.  
Specimen Preparation
Blood: Transport 1 mL whole blood in heparin, sodium oxalate tube, or blood culture bottles. (Min: 1 mL)
CSF:
Transport 0.5 mL CSF in a sterile container. (Min: 0.1 mL)
Urine:
Dilute 1 mL urine 1:10 in 1 percent bovine serum albumin within one hour of collection. Transport 4 mL diluted urine in a sterile container. (Min: 0.1 mL)  
Storage/Transport Temperature
Room temperature.  
Unacceptable Conditions
Clotted whole blood or whole blood submitted in anticoagulant other than those listed above. Urine not diluted with bovine serum albumin within 1 hour of collection or urine in preservative.  
Remarks
Specimen source required. Leptospira spp. may not survive extended transport time or in acidic urine.  
Stability
Blood culture bottles: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Other:
Ambient 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
Culture negative for Leptospira.
Interpretive Data
Note
Identification and susceptibility tests are billed separately from culture.
CPT Code(s)
87081; 87166
Components
Component Test Code*Component Chart Name
0060158Culture, Leptospira
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Culture, Leptospira