Clostridium difficile Culture with Reflex to Cytotoxin Cell Assay
0060140
Ordering Recommendation
Reference method for diagnosis of C. difficile-associated diarrhea (turnaround time ≥96 hrs). Preferred initial test is C. difficile toxin B gene (tcdB) by PCR.
Mnemonic
MC CDIF
Methodology
Culture/Identification
Performed
Sun-Sat
Reported
Within 3 days
Preliminary: Negative at 2 days or as soon as positive detected
Final: Negative at 3 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Stool: Transfer 5 mL to an unpreserved stool transport vial (ARUP supply #40910). OR place 5 mL in enteric transport media (Cary-Blair) (ARUP supply #29799). (Min: 1 mL) Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Tissue:
 Transfer specimen immediately into anaerobe transport media (ARUP supply #16426).  
Specimen Preparation
Transfer 5 mL stool to an unpreserved stool transport vial (ARUP supply #40910). OR place 5 mL stool in enteric transport media (Cary-Blair) (ARUP supply #29799). (Min: 1 mL) Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Storage/Transport Temperature: Frozen.  
Storage/Transport Temperature
Stool: Frozen. Tissue: Room Temperature.  
Unacceptable Conditions
Multiple specimens (more than one in 24 hours), formed stools, or stool in PVA or formalin.  
Remarks
  
Stability
Unpreserved or preserved Stool: Ambient: 2 hours; Refrigerated: 48 hours; Frozen: 1 week. Tissue: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.  
Reference Interval
Culture negative for Clostridium difficile.  
Interpretive Data
 
Note
For routine stool screening, refer to Clostridium difficile toxin B gene (tcdB) by PCR (ARUP test code 2002838).

If Clostridium difficile culture is positive, then Clostridium difficile Cytotoxin Cell Assay (ARUP test code 0060851) will be added. Additional charges apply.
CPT Code(s)
87075; if reflexed add 87230
Components
Component Test Code*Component Chart Name
0060140Culture, Clostridium difficile
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • C. diff culture
  • C. difficile Culture
  • Culture, Clostridium difficile