Hepatitis B Virus by Quantitative PCR
0056025
Ordering Recommendation
 
Mnemonic
HBVUPCR
Methodology
Quantitative Polymerase Chain Reaction
Performed
Sun-Sat
Reported
2-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA), pink (K2EDTA) or serum separator tube.  
Specimen Preparation
Separate serum or plasma from cells within 24 hours. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 1 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
Heparinized specimens.  
Remarks
  
Stability
On cells: Ambient: 24 hours. After separation from cells: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 weeks  
Reference Interval
Not detected  
Interpretive Data
The quantitative range of this assay is 1.3-8.2 log IU/mL (20-170,000,000 IU/mL).
1 IU/mL of HBV DNA is approximately 5.82 copies/mL.

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).
 
Note
The limit of quantification for this DNA assay is 1.3 log IU/mL (20 IU/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<1.3 log IU/mL (<20 IU/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies, the test result will be reported as "Not Quantified."

Specimens received with less than minimum volume for testing will automatically be run with a dilution according to the guidelines below:
-Specimens with 325-650 µL will be diluted resulting in a modification of the quantitative range of the assay to 1.6-8.5 log IU/mL (41-350,000,000 IU/mL).
-Specimens with 130-325 µL will be diluted resulting in a modification of the quantitative range of the assay to 2.0-8.9 log IU/mL (102-870,000,000 IU/mL).

This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to treatment as measured by changes in HBV DNA levels.
CPT Code(s)
87517
Components
Component Test Code*Component Chart Name
0051820HBV Qnt by PCR Interpretation
0056026HBV Qnt by PCR (log IU/mL)
2002322HBV Qnt by PCR (IU/mL)
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HBV Quant PCR
  • HBV quantitative
  • HBV Viral Load
  • Nucleic Acid Amplification Test (NAAT)
  • Viral load monitoring