Vitamin B12 with Reflex to Methylmalonic Acid, Serum (Vitamin B12 Status)
0055662
 
Ordering Recommendation
Preferred reflex test for detection of vitamin B12 deficiency in individuals with macrocytic or unexplained anemia, or unexplained neurologic disease.
Mnemonic
B12 MMA
Methodology
Quantitative Chemiluminescent Immunoassay/Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Plain red or serum separator tube. Also acceptable: Green (sodium or lithium heparin).  
Specimen Preparation
Protect from light during collection, storage and shipment. Centrifuge and remove serum from cells within 2 hours of collection. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube and refrigerate or freeze immediately. (Min: 1.5 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
EDTA plasma. Room temperature specimens. Grossly hemolyzed or lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: Unacceptable; Refrigerated: 48 hours; Frozen: 1 month  
Reference Interval
Available Separately Components Reference Interval
Yes (0070150) Vitamin B12 Effective December 2, 2013
180-​914 pg/mL
No Methylmalonic Acid, Serum (Vitamin B12 Deficiency) 0.00-​0.40 µmol/L
Interpretive Data
Note
If Vitamin B12 is less than 300 pg/mL, then Methylmalonic Acid, Serum (Vitamin B12 Status) will be added. Additional charges apply.
CPT Code(s)
82607; if reflexed, add 83921
Components
Component Test Code*Component Chart Name
0055663Vitamin B12 Status
0055664Methylmalonic Acid, Serum
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • B12 (Cobalamin)
  • B12 and MMA reflex
  • B12 Assay
  • B12 MMA
  • B12, Serum
  • CblC
  • Cobalamin
  • Methylcobalamin
  • Vit B12 and MMA