Human Immunodeficiency Virus 1 by Quantitative PCR
0055598
Ordering Recommendation
 
Mnemonic
HIVPCRQ
Methodology
Quantitative Polymerase Chain Reaction
Performed
Sun-Sat
Reported
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA), pink (K2EDTA), plasma preparation tube, or CSF.  
Specimen Preparation
Separate plasma from cells within 24 hours of collection. Transfer 3 mL plasma or CSF to an ARUP Standard Transport Tube and freeze. (Min: 1.5 mL)  
Storage/Transport Temperature
Frozen.   
Unacceptable Conditions
Serum. Heparinized specimens. Specimens submitted in plasma preparation tube.  
Remarks
  
Stability
On Cells: Ambient: 24 hours; After separation from cells: Ambient: 24 hours; Refrigerated: 6 days; Frozen: 6 weeks  
Reference Interval
Not detected  
Interpretive Data
The quantitative range of this assay is 1.3-7.0 log copies/mL (20-10,000,000 copies/mL).

An interpretation of "Not Detected" does not rule out the presence of PCR inhibitors or HIV-1 virus RNA concentrations below the level of detection of the assay. Care should be taken in the interpretation of any single viral load determination. The clinical significance of changes in HIV-1 RNA measurements has not been fully established; however, a change of 0.5 log copies/mL may be significant.

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).  
Note
The limit of quantification for this RNA assay is 1.3 log copies/mL (20 copies/mL). If the assay DID NOT DETECT the virus, the test result will be reported as "<1.3 log copies/mL (<20 copies/mL)." If the assay DETECTED the presence of the virus but was not able to accurately quantify the number of copies the test result will be reported as "Not Quantified."

Specimens received with less than minimum volume for testing will automatically be run with a dilution according to the guidelines below:
--Specimens with 500-1000 µL will be diluted resulting in a modification of the quantitative range of the assay to 1.6-7.3 log copies/mL (41-20,000,000 copies/mL).
--Specimens with 200-500 µL will be diluted resulting in a modification of the quantitative range of the assay to 2.0-7.7 log copies/mL (102-51,000,000 copies/mL).

This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in HIV-1 RNA levels.
CPT Code(s)
87536
Components
Component Test Code*Component Chart Name
0020297HIV-1 Qnt by PCR (log copy/mL)
0051817HIV-1 Qnt by PCR Interp
2002646HIV-1 Qnt by PCR (copy/mL)
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • HIV quantitative
  • HIV-1 RNA
  • HIV-1 RNA Viral Load
  • HIV1 viral load monitoring
  • Human Immunodeficiency Virus 1 (Abbott RealTime)
  • Nucleic Acid Amplification Test (NAAT)