Hepatitis C Virus Genotype by Sequencing
0055593
 
Ordering Recommendation
Do not order prior to molecular confirmation of positive hepatitis C virus (HCV) screen. Order before initiating HCV therapy to aid in determining therapy of choice, likelihood of response, and probable therapeutic duration. Assay does not subtype.
Mnemonic
HEPCGENO
Methodology
Polymerase Chain Reaction/Sequencing
Performed
Sun-Sat
Reported
3-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), plasma preparation tube or serum separator tube.  
Specimen Preparation
Separate serum or plasma from cells within 6 hours. Transfer 2 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
Heparinized specimens.  
Remarks
Please submit most recent viral load and test date, if available.  
Stability
On cells: Ambient: 6 hours. After separation from cells: Refrigerated: 72 hours; Frozen: 4 months.  
Reference Interval
By report
Interpretive Data
Hepatitis C viral RNA is tested using reverse transcription polymerase chain reaction (RT-PCR) to amplify a specific portion of the 5' untranslated region (5' UTR) of the viral genome. The amplified nucleic acid is sequenced bi-directionally using dye-terminator chemistry (ABI). Sequencing data is compared to a database of characterized sequences.

Isolates of hepatitis C virus are grouped into six major genotypes (1-6). These genotypes are subtyped according to sequence characteristics. Due to high conservation of the 5' un-translated region of the HCV genome, this test has limitations in differentiating subtype 1a from 1b. Therefore, these subtypes will be reported as 1a or 1b. In rare instances, Type 6 virus may be misclassified as Type 1.

See Compliance Statement B: www.aruplab.com/CS
Note
This test may be unsuccessful if the HCV RNA viral load is less than log 2.8 or 600 IU/mL.
CPT Code(s)
87902
Components
Component Test Code*Component Chart Name
0055593HCV Genotype by Sequencing
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • HCV 5'UTR Genotype
  • HCV 5'UTR Sequencing
  • HCV Genotype
  • HCV Genotyping
  • HCV Sequencing
  • HCV, Subtype