Leptospira Antibody, IgM by Dot Blot
0055233
Ordering Recommendation
Submit With Order
Mnemonic
LEPTO M
Methodology
Qualitative Immunoblot
Performed
Mon, Thu
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.05 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Please mark specimen plainly as acute or convalescent.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, severely lipemic specimens. Any other body fluid.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Negative: No significant level of Leptospira IgM antibody detected.
Equivocal: Questionable presence of Leptospira IgM antibody detected. Repeat testing in 10-14 days may be helpful.
Positive: Presence of IgM antibody to Leptospira detected, suggestive of a current or recent infection.
Interpretive Data
Samples interpreted as negative indicate that antibody is not present in the sample, or is below the detection level of the method. Since antibodies may not be present during early disease, confirmation two to three weeks later is recommended. An initially-negative result followed by a positive result indicates IgM seroconversion.

Equivocal specimens should be cautiously interpreted. Further testing with an additional specimen is recommended. If the specimen remains equivocal, a second serological method should be considered if leptospirosis infection is still suspected.

Samples interpreted as positive may indicate the specific antibody. Antibody presence alone cannot be used for diagnosis of acute infection, however, because antibodies from prior exposure may circulate for a prolonged period of time.
Note
A negative result does not rule out the possibility of leptospirosis.
CPT Code(s)
86720
Components
Component Test Code*Component Chart Name
0055233Leptospira IgM
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Cross References
  • Leptospira IgM antibody
  • Leptospira IgM Dot Blot