Hepatitis C Virus RNA Quantitative bDNA
0051811
Ordering Recommendation
Not recommended to confirm active hepatitis C virus (HCV) infection. Hepatitis C Virus by Quantitative PCR (0098268) is the preferred test. following a positive HCV screen.
Mnemonic
HCVRNA BC
Methodology
Quantitative Branched Chain DNA
Performed
Mon, Thu
Reported
3-8 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA), pink (K2EDTA), plasma preparation tube, or serum separator tube.  
Specimen Preparation
Separate serum or plasma from cells within 4 hours. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.2 mL).
PPT:
Separate plasma from cells within 2 hours. Transport 1 mL plasma in original container. (Min: 0.2 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
Heparinized specimens.  
Remarks
  
Stability
PPT: On Cells: Ambient: 2 hours. All others: On cells: Ambient 4 hours. After separation from cells; Refrigerated: 48 hours; Frozen at -20°C: 72 hours; Frozen at -70°C: 4 months  
Reference Interval
Not detected  
Interpretive Data
The quantitative range of this assay is 2.8-6.9 log IU/mL (615-7,690,000 IU/mL).

An interpretation of "Not Detected" does not rule out the presence of inhibitors in the patient specimen or hepatitis C virus RNA concentrations below the level of detection of the assay. Care should be taken in the interpretation of any single viral load determination. Low positive values may occasionally be seen in specimens from patients who are uninfected.

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).
 
Note
This test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in HCV RNA levels. A quantitative PCR assay with a detection limit of 1.2 log IU/mL (18 IU/mL), Hepatitis C Virus RNA Quantitative, Real-Time PCR (0098268), is available for the assessment of lower levels of HCV viremia.
CPT Code(s)
87522
Components
Component Test Code*Component Chart Name
0051812HCV RNA Quant bDNA Interpretation
0051823HCV RNA Quant bDNA, Log
2002645HCV RNA Quant bDNA, IU
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Aliases
  • HCV branch DNA
  • HCV quantitative
  • HCV Quantitative bDNA
  • HCV viral load
  • Signal amplification
  • Viral load monitoring