QuantiFERON TB Gold In-Tube
0051729
 
Ordering Recommendation
Mnemonic
QFTG-TB IT
Methodology
Cell Culture/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
At altitudes from sea level to 2,650 feet: QuantiFERON TB (Standard) Gold In-Tube collection kit.
At altitudes from 2,650-3,350 feet:
QuantiFERON TB (Standard) Gold In-Tube collection kit.
At altitudes from 3,350-6,150 feet:
QuantiFERON TB (High Altitude) Gold In-Tube collection kit.
At altitudes above 6,150 feet:
QuantiFERON TB (High Altitude) Gold In-Tube collection kit.  
Specimen Preparation
Specimen Preparation: At altitudes from sea level to 2,650 feet: Whole blood directly into each of the three tubes in the QuantiFERON-TB (Standard) Gold In-Tube collection
it (ARUP supply #45112) available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787.
At altitudes from 2,650-3,350 feet:
Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON-TB
Standard) Gold In-Tube collection kit (ARUP supply #45112).
At altitudes from 3,350-6,150 feet:
Whole blood directly into each of the three tubes in the QuantiFERON-TB (High Altitude) Gold In-Tube collection kit (ARUP supply
46261) available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787.
At altitudes above 6,150 feet:
Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON-TB (High
Altitude) Gold In-Tube collection kit (ARUP supply #46261).Tubes should be between 17°C and 25°C (63°F to 77°F) at the time of blood
filling.
Immediately after filling tubes, shake them ten (10) times just firmly enough to ensure the entire inner surface of the tube is coated with blood, to solubilize antigens on tube
walls. Over-energetic shaking may cause gel disruption and could lead to aberrant results.
After mixing,
incubate upright at 37°C for 16-24 hours (within 16 hours of collection).
After incubation,
centrifuge for 15 minutes at 2000-3000 RCF (g).
After centrifugation,
transport entire contents in each of the 3 original collection tubes.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Specimens that have not been incubated and centrifuged.  
Remarks
Further information on mixing QuantiFERON TB Gold In-Tube collection tubes and an instructional video can be found at: http://www.aruplab.com/Specimen-Handling/SpecialSpecimenCollection/quantiferon.jsp  
Stability
After incubation and centrifugation: Ambient: 2 hours; Refrigerated: 4 weeks; Frozen: Unacceptable  
Reference Interval
QuantiFERON TB Gold In-​Tube
Effective November 19, 2012
Components Reference Interval
QuantiFERON-​TB Gold In-​Tube Negative
QuantiFERON-​TB minus NIL 0.34 IU/mL or less
QuantiFERON MITOGEN minus NIL No reference interval
QuantiFERON NIL No reference interval
Interpretive Data
The QuantiFERON software interprets the three values, NIL, MITOGEN minus NIL (MITOGEN-NIL), and TB minus NIL (TB-NIL), to provide the qualitative result (Negative, Positive, or Indeterminate). The NIL value represents nonspecific reactivity detected within the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB-NIL value represents lymphocyte reactivity specifically stimulated by the TB antigen. An overall Negative result does not completely rule out TB infection. An overall Positive result does not differentiate active from latent tuberculosis.
Note
The ability of the QuantiFERON TB Gold In-Tube assay to predict the progression of latent tuberculosis infection to active tuberculosis has not been evaluated. For questions about collection and stability of sample please call Microbial Immunology 1 at (801) 583-2787, extension 2270.

Further information on mixing QuantiFERON TB Gold In-Tube collection tubes and an instructional video can be found at www.aruplab.com/quantiferon
CPT Code(s)
86480
Components
Component Test Code*Component Chart Name
0051728QuantiFERON- TB Gold In-Tube
2006961QuantiFERON- TB minus NIL
2006962QuantiFERON Mitogen minus NIL
2006963QuantiFERON NIL
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Interferon-Gamma Release Assay for Tuberculosis
  • Latent Tuberculosis Infection (LTBI)
  • QFT & M TB
  • QFT-G & M. tuberculosis
  • QFT-TB
  • Quantiferon Mitogen and NIL
  • QuantiFERON-TB Gold In Tube & Mycobacterium tuberculosis Antibody