QuantiFERON-TB Gold In-Tube
0051729
Ordering Recommendation
 
Mnemonic
QFTG-TB IT
Methodology
Cell Culture/Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Sun-Sat
Reported
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
At altitudes from sea level to 2,650 feet: QuantiFERON TB (Standard) Gold In-Tube collection kit.
At altitudes from 2,650-3,350 feet:
QuantiFERON TB (Standard) Gold In-Tube collection kit.
At altitudes from 3,350-6,150 feet:
QuantiFERON TB (High Altitude) Gold In-Tube collection kit.
At altitudes above 6,150 feet:
QuantiFERON TB (High Altitude) Gold In-Tube collection kit.  
Specimen Preparation
Specimen Preparation: At altitudes from sea level to 2,650 feet: Whole blood directly into each of the three tubes in the QuantiFERON-TB (Standard) Gold In-Tube collection
it (ARUP supply #45112) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes from 2,650-3,350 feet:
Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON-TB
Standard) Gold In-Tube collection kit (ARUP supply #45112).
At altitudes from 3,350-6,150 feet:
Whole blood directly into each of the three tubes in the QuantiFERON-TB (High Altitude) Gold In-Tube collection kit (ARUP supply
46261) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
At altitudes above 6,150 feet:
Collect 3 mL whole blood using a syringe. Transfer 1 mL (Min: 0.8 mL, Max 1.2 mL) to each of the three tubes in QuantiFERON-TB (High
Altitude) Gold In-Tube collection kit (ARUP supply #46261).Tubes should be between 17°C and 25°C (63°F to 77°F) at the time of blood
filling.
Immediately after filling tubes, shake them ten (10) times just firmly enough to ensure the entire inner surface of the tube is coated with blood, to solubilize antigens on tube
walls. Over-energetic shaking may cause gel disruption and could lead to aberrant results.
After mixing,
incubate upright at 37°C for 16-24 hours (within 16 hours of collection).
After incubation,
centrifuge for 15 minutes at 2000-3000 RCF (g).
After centrifugation,
transport entire contents in each of the 3 original collection tubes.  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Specimens that have not been incubated and centrifuged.  
Remarks
Further information on mixing QuantiFERON TB Gold In-Tube collection tubes and an instructional video can be found at: http://www.aruplab.com/Specimen-Handling/SpecialSpecimenCollection/quantiferon.jsp  
Stability
After incubation and centrifugation: Ambient: 2 hours; Refrigerated: 4 weeks; Frozen: Unacceptable  
Reference Interval
 
 
QuantiFERON TB Gold In-​Tube
Effective November 19, 2012
Components Reference Interval
QuantiFERON-​TB Gold In-​Tube Negative
QuantiFERON-​TB minus NIL 0.34 IU/mL or less
QuantiFERON MITOGEN minus NIL No reference interval
QuantiFERON NIL No reference interval
Interpretive Data
Interferon gamma release is measured for specimens from each of the three collection tubes. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the three values, NIL, MITOGEN minus NIL (MITOGEN-NIL), and TB minus NIL (TB-NIL). The NIL value represents nonspecific reactivity produced by the patient specimen. The MITOGEN-NIL value serves as the positive control for the patient specimen, demonstrating successful lymphocyte activity. The TB-NIL value represents lymphocyte reactivity specifically stimulated by the TB antigen. An overall Negative result does not completely rule out TB infection.

A false-positive result in the absence of other clinical evidence of TB infection is not uncommon. Refer to: Updated Guidelines for Using Interferon Gamma Release Assays to Detect Mycobacterium tuberculosis Infection---United States, 2010 (http://www.cdc.gov/mmwr/preview/mmwrhtml/rr5905a1.htm), for information concerning test performance in low-prevalence populations and use in occupational screening.  
Note
The ability of the QuantiFERON TB Gold In-Tube assay to predict the progression of latent tuberculosis infection to active tuberculosis has not been evaluated.  For questions about collection and stability of sample please call Microbial Immunology 1 at (801) 583-2787, extension 2270. 

Further information on mixing QuantiFERON TB Gold In-Tube collection tubes and an instructional video can be found at www.aruplab.com/quantiferon
CPT Code(s)
86480
Components
Component Test Code*Component Chart NameLOINC
0051728QuantiFERON- TB Gold In-Tube45323-3
2006961QuantiFERON- TB minus NIL39017-9
2006962QuantiFERON Mitogen minus NIL46216-8
2006963QuantiFERON NIL71774-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Interferon-Gamma Release Assay for Tuberculosis
  • Latent Tuberculosis Infection (LTBI)
  • QFT & M TB
  • QFT-G & M. tuberculosis
  • QFT-TB
  • Quantiferon Mitogen and NIL
  • QuantiFERON-TB Gold In Tube & Mycobacterium tuberculosis Antibody