Ordering Recommendation

Do not use to screen for celiac disease.  May be used to estimate severity of intestinal villous atrophy in confirmed celiac disease.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Transfer 0.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions

Plasma. Contaminated, severely hemolyzed, icteric, or lipemic specimens.

Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year

Methodology

Semi-Quantitative Enzyme-Linked Immunosorbent Assay

Performed

Tue

Reported

1-8 days

Reference Interval

Test Number
Components
Reference Interval
  F-Actin Antibody, IgA 24.9 or less

Interpretive Data

The presence of IgA antibodies against F-Actin in patients having positive serologic markers for celiac disease, such as antiendomysial, anti-transglutaminase, and/or anti-gliadin peptide IgA, indicates the presence of intestinal villus atrophy. Positive results should be confirmed with biopsy.


Component
Interpretation
F-Actin (Smooth Muscle) Antibody, IgA 20.0 Units or less              Negative
20.1-24.9                          Equivocal
25.0 Units or greater         Positive

Compliance Category

FDA

Note

Hotline History

N/A

CPT Codes

86015

Components

Component Test Code* Component Chart Name LOINC
0051725 F-Actin Antibody, IgA 63210-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • ACTA
  • Actin Antibody
  • Anti-Smooth Muscle Antibody
  • ASMA
  • F-Actin Antibody
  • SMA
F-Actin (Smooth Muscle) Antibody, IgA