Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, TH2 Cytokines (INACTIVE as of 04/04/16)
Ordering Recommendation
Cell Culture/Multiplex Bead Assay
9-10 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient. 
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test performance 
Specimen Preparation
Transport 20 mL whole blood (patient) AND 20 mL whole blood (control) in original collection tubes. (Min: 14 mL (patient) AND 14 mL (control)) Do not refrigerate or freeze. LIVE CELLS REQUIRED.
Infant Minimum: 3 mL (patient) AND 14 mL (control). 
Storage/Transport Temperature
Unacceptable Conditions
Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours. 
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
Available Separately
Associated Tests
Reference Interval
0096056Lymphocyte Antigen and Mitogen Proliferation PanelBy report
0051576Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 4 (INACTIVE as of 04/04/16)By report
0051577Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 5 (INACTIVE as of 04/04/16)By report
0051578Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 10 (INACTIVE as of 04/04/16)By report
0051579Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 13 (INACTIVE as of 04/04/16)By report

Interpretive Data
Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection.

Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s)
86353 x5; 83520 x4
Component Test Code*Component Chart NameLOINC
0051586Lymphocyte Proliferation, TH2 Cytokine
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Blastogenesis Antigens
  • Blastogenesis Mitogens
  • IL-10
  • IL-13
  • IL-4
  • IL-5
  • IL12
  • IL2
  • IL2R
  • Lymphocyte Blastogenesis Antigen
  • Lymphocyte Blastogenesis Mitogens
  • Mitogen Studies
  • Phytohemagglutinin