Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, TH1 Cytokines
0051585
Ordering Recommendation
 
Mnemonic
LAM TH1 CY
Methodology
Cell Culture/Multiplex Bead Assay
Performed
Tue-Fri
Reported
9-10 days  
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Time SensitiveTime Sensitive

Specimen Required
Patient Preparation
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.  
Collect
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test performance.  
Specimen Preparation
Transport 20 mL whole blood (patient) AND 20 mL whole blood (control) in original collection tubes. (Min: 14 mL (patient) AND 14 mL (control)) Do not refrigerate or freeze. LIVE CELLS REQUIRED.
Infant Minimum: 3 mL (patient) AND 14 mL (control).  
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE.  
Unacceptable Conditions
Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.  
Remarks
 
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients:
Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
 
 
Available Separately Associated Tests Reference Interval
0096056Lymphocyte Antigen and Mitogen Proliferation Panel By report
0051571Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 By report
0051572Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 Receptor (CD25), Soluble By report
Interpretive Data
Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection.

Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

See Compliance Statement B: www.aruplab.com/CS
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s)
86353 x5; 83520 x4
Components
Component Test Code*Component Chart NameLOINC
0051585Lymphocyte Proliferation, TH1 Cytokine 
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • (PHA) Phytohemagglutinin
  • Blastogenesis Antigens
  • Blastogenesis Mitogens
  • IFN Gamma
  • IL-12
  • IL-2
  • IL-2R
  • Immune Competence
  • Lymphocyte Blastogenesis Antigen
  • Lymphocyte Blastogenesis Mitogens
  • Lymphocyte Phytohemagglutinin
  • Lymphocyte Transformation
  • Mitogen Studies
  • Mitogens
  • Pokeweed (PWM)