Lymphocyte Antigen and Mitogen Proliferation Panel with Cytokine Response to Mitogens, 12 Cytokines
0051584
Ordering Recommendation
 
Mnemonic
LAM CYT 12
Methodology
Cell Culture/Multiplex Bead Assay
Performed
Tue-Fri
Reported
9-10 days  
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Time SensitiveTime Sensitive

Specimen Required
Patient Preparation
Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.  
Collect
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD solution A) (patient) AND yellow (ACD solution A) (control). Patient and control specimens must be collected within 48 hours of test  
Specimen Preparation
Transport 20 mL whole blood (patient) AND 20 mL whole blood (control) in original collection tubes. (Min: 14 mL (patient) AND 14 mL (control)) Do not refrigerate or freeze. LIVE CELLS REQUIRED.
Infant Minimum: 3 mL (patient) AND 14 mL (control).  
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE.  
Unacceptable Conditions
Yellow (ACD Solution B). Refrigerated or frozen specimens. Specimens in transport longer than 48 hours.  
Remarks
 
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients:
Ambient 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
 
 
Test Number Components Reference Interval
0096056Lymphocyte Antigen and Mitogen Proliferation Panel By report
0051574Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interferon gamma By report
0051580Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 1 beta By report
0051571Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 By report
0051572Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 2 Receptor (CD25), Soluble By report
0051576Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 4 By report
0051577Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 5 By report
0051581Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 6 By report
0051582Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 By report
0051578Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 10 By report
0051579Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 13 By report
0051583Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Tumor Necrosis Factor alpha By report
Interpretive Data
Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in mononuclear cell culture. Cell proliferation in response to mitogens and antigens is determined by 3H-thymidine incorporation. Cytokine production in response to mitogens is determined by multi-analyte fluorescence detection.

Proliferation results are reported as the mitogen and/or antigen stimulated counts per minute (CPM) and a stimulation index (SI) which represents the ratio of the stimulated lymphocyte CPM to an unstimulated control CPM. Cytokine results are reported in pg/mL.

Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders

See Compliance Statement B: www.aruplab.com/CS  
Note
Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s)
86353 x5; 83520 x12
Components
Component Test Code*Component Chart Name
0051584Lymphocyte Proliferation, Cytokine 12
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Blastogenesis Antigens
  • Blastogenesis Mitogens
  • IFN Gamma
  • IFND
  • IL 1 beta
  • IL 10
  • IL 12 Receptor
  • IL 13
  • IL 2
  • IL 4
  • IL 5
  • IL 6
  • IL 8
  • IL-1 Beta
  • IL-10
  • IL-12
  • IL-13
  • IL-2
  • IL-2R
  • IL-4
  • IL-5
  • IL-6
  • IL-8
  • IL2 Receptor
  • Lymphocyte Blastogenesis Antigen
  • Lymphocyte Blastogenesis Mitogens
  • Mitogen Studies
  • Phytohemagglutinin
  • TNF Alpha
  • TNFa