Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 8 (INACTIVE as of 04/04/16)
Ordering Recommendation
Cell Culture/Quantitative Multiplex Bead Assay
9-10 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen. 
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control samples must be collected within 48 hours of test performance. 
Specimen Preparation
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL whole blood (patient) AND 7 mL whole blood (control)). LIVE CELLS REQUIRED. Do not refrigerate or freeze.
Infant Minimum:
3 mL whole blood (patient) AND 7 mL whole blood (control). 
Storage/Transport Temperature
Unacceptable Conditions
Yellow (ACD Solution B). Specimens in transport longer than 48 hours. 
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
New York State Clients: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data
Phytohemagglutinin, concanavalin A, and pokeweed mitogen are tested independently in lymphocyte culture. Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by multi-analyte fluorescence detection. Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s)
86353 x3; 83520
Component Test Code*Component Chart NameLOINC
0051582Cytokine Production, IL-8 Response
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • IL-8
  • IL8