Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, TH2 Cytokines
0051562
 
Time SensitiveTime Sensitive
Ordering Recommendation
Mnemonic
CYT TH2 LY
Methodology
Cell Culture/Quantitative Multiplex Bead Assay
Performed
Tue-Fri
Reported
9-10 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
A control specimen needs to be sent with the patient specimen. The control specimen needs to be drawn from a normal, healthy individual who is not biologically related to the patient, and drawn at approximately the same time as and under similar conditions to the patient specimen.  
Collect
Green (sodium heparin) (patient) AND green (sodium heparin) (control). Also acceptable: Yellow (ACD Solution A) (patient) AND yellow (ACD Solution A) (control). Patient and control samples must be collected within 48 hours of test performance.  
Specimen Preparation
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL whole blood (patient) AND 7 mL whole blood (control)). LIVE CELLS REQUIRED. Do not refrigerate or freeze.
Infant Minimum:
3 mL whole blood (patient) AND 7 mL whole blood (control).  
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE.  
Unacceptable Conditions
Yellow (ACD Solution B). Specimens in transport longer than 48 hours.  
Remarks
 
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
Test Number Components Reference Interval
0051576Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 4 By report
0051577Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 5 By report
0051578Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 10 By report
0051579Cytokine Production by Mononuclear Cells in Response to Mitogen Stimulation, Interleukin 13 By report
Interpretive Data
Phytohemagglutinin, concanavalin A, and pokeweed mitogen are tested independently in lymphocyte culture. Peripheral Blood Mononuclear Cell (PBMC) cytokine responses to these mitogens are determined by multi-analyte fluorescence detection. Results are to be used for research purposes or in attempts to understand the pathophysiology of immune, infectious, or inflammatory disorders.

See Compliance Statement B: www.aruplab.com/CS
Note
Results are reported as pg/mL. Interpretation comparing the patient results to the simultaneously collected client normal control and the laboratory normal control will be provided by an ARUP medical director.
CPT Code(s)
86353 x3; 83520 x4
Components
Component Test Code*Component Chart Name
0051576Cytokine Production, IL-4 Response
0051577Cytokine Production, IL-5 Response
0051578Cytokine Production, IL-10 Response
0051579Cytokine Production, IL-13 Response
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • IL-10
  • IL-13
  • IL-4
  • IL-5
  • IL10
  • IL13
  • IL4
  • IL5