Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Serotonin Release Assay
This test is New York DOH approved.
- Patient Preparation
- Plain red or serum separator tube (SST).
- Specimen Preparation
- Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 3 mL serum to an ARUP Standard Transport Tube. (Min: 2 mL) Also acceptable: ACD or sodium citrated plasma.
- Storage/Transport Temperature
- CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Ship on dry ice.
- Unacceptable Conditions
- Heparinized specimens.
- Microbially contaminated, heat-inactivated, hemolyzed, icteric, or lipemic sera may give inconsistent results.
- After separation from cells: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 month
Effective November 14, 2011
|0051052||Heparin-Induced Thrombocytopenia (HIT) Antibodies, PF4 IgG/IgM/IgA by ELISA
||0.399 OD or less
|2005631||Serotonin Release Assay (Heparin Dependent Platelet Antibody), Unfractionated Heparin
By itself, a positive result on this ELISA test is relatively non-specific for a clinical diagnosis of heparin-associated antibody syndrome (HIT) (frequent false-positives) and a negative result does not exclude a diagnosis of HIT if the clinical suspicion remains high (occasional false-negatives). Results should be used in conjunction with clinical findings, platelet counts and other laboratory results.
For a clinical scoring system to assess pretest probability of HIT and other guidance for diagnosing HIT, refer to the Heparin-Associated Antibody Syndrome topic at arupconsult.com.
Positive ELISA results are relatively non-specific for a clinical diagnosis of HIT. If ELISA result is positive, then Serotonin Release Assay (a functional assay with greater specificity for a clinical diagnosis of HIT syndrome) will be added. Additional charges apply.
86022; if reflexed add 86022
|Component Test Code*||Component Chart Name|
|0051052||Heparin-Induced Thrombocytopenia Abs PF4|
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at email@example.com.
- Heparin PF4 Antibody
- Heparin-Associated Antibody
- Type II HIT