EWSR1-FLI1 and EWSR1-ERG Translocations by RT-PCR
0051220
Ordering Recommendation
Confirm histological diagnosis of Ewing sarcoma by detection of EWSR1-FLI1 and EWSR1-ERG fusions.
Submit With Order
Mnemonic
EWS
Methodology
Reverse Transcription Polymerase Chain Reaction
Performed
RNA Isolation: Sun-Sat; Assay: Varies
Reported
3-7 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Tissue.  
Specimen Preparation
Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 fresh tissue in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Also acceptable: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 4 tissue scrolls (tissue shavings), 10 microns thick in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. Protect paraffin block from excessive heat. Tissue block will be returned after testing.  
Storage/Transport Temperature
Fresh Tissue: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
FFPE:
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Slides. Decalcified tissue. Paraffin section slides.  
Remarks
 
Stability
Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 year
FFPE:
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
By report
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
81401 x2
Components
Component Test Code*Component Chart Name
0051219EWSR1-FLI1 and EWSR1-ERG by RT-PCR
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • ERG
  • ewing sarcoma breakpoint
  • EWS
  • EWS-FL1 Type 1
  • EWS-FLI1 type 2
  • EWSC
  • EWSR1-FL1 type 1
  • EWSR1-FL1 type 2
  • FLI1
  • FLI1 type 1
  • FLI1 type 2
  • FLI2
  • t(21;22)