Treponema pallidum Antibody, IgM by ELISA (Temporary Delay as of 10/16/14 - contact Client Services for referral options)
0050921
Ordering Recommendation
Aids in the workup of suspected congenital syphilis. May use to confirm reactive nontreponemal syphilis screening test. May also be used as a primary syphilis screening test in so-called reverse screening algorithm.
Mnemonic
SYPH M
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Wed
Reported
1-8 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.05 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
0.8 IV or less: Negative - No significant level of Treponema pallidum IgM antibody detected.
0.9-1.1 IV: Equivocal - Questionable presence of Treponema pallidum IgM antibody detected.  Repeat testing 10-14 days may be helpful.
1.2 IV or greater: Positive - Presence of IgM antibody to Treponema pallidum detected, suggestive of current or recent infection.  
Interpretive Data
The CDC recommends initial syphilis screening using a non-treponemal test, such as RPR, prior to confirmation with a treponemal method such as this ELISA or TP-PA.

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are performed in the same laboratory at the same time. IgM testing is a useful diagnostic aid in differentiating maternally derived IgG antibodies that cross the placenta from IgM antibodies of an active infection in the newborn. Sensitivity in IgM testing for congenital syphilis is 80 percent, therefore, a negative IgM does not necessarily rule out the possibility of congenital syphilis.  
Note
 
CPT Code(s)
86780
Components
Component Test Code*Component Chart Name
0050921T.Pallidum Antibody, IgM by ELISA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Syphilis Antibody
  • Syphilis IgM ELISA
  • T pallidum IgM by ELISA