- Patient Preparation
- Serum separator tube.
- Specimen Preparation
- Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.05 mL)
- Storage/Transport Temperature
- Unacceptable Conditions
- Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.
- After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
0.9-1.1 IV: Equivocal - Questionable presence of Treponema pallidum IgM antibody detected. Repeat testing 10-14 days may be helpful.
1.2 IV or greater: Positive - Presence of IgM antibody to Treponema pallidum detected, suggestive of current or recent infection.
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are performed in the same laboratory at the same time. IgM testing is a useful diagnostic aid in differentiating maternally derived IgG antibodies that cross the placenta from IgM antibodies of an active infection in the newborn. Sensitivity in IgM testing for congenital syphilis is 80 percent, therefore, a negative IgM does not necessarily rule out the possibility of congenital syphilis.
|Component Test Code*||Component Chart Name|
|0050921||T.Pallidum Antibody, IgM by ELISA|
- Syphilis Antibody
- Syphilis IgM ELISA
- T pallidum IgM by ELISA