Treponema pallidum Antibody, IgG by ELISA
0050920
Ordering Recommendation
Recommended syphilis screening test in so-called reverse screening algorithm. The CDC recommends a nontreponemal test (RPR or VDRL) for initial syphilis screening prior to confirmation with a treponemal test (eg, TP-PA).
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Mnemonic
SYPH G
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Wed
Reported
1-8 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.05 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
0.8 IV or less: Negative - No significant level of Treponema pallidum IgG antibody detected.
0.9-1.1 IV: Equivocal - Questionable presence of Treponema pallidum IgG antibody detected. Repeat testing in 10-14 days may be helpful.
1.2 IV or greater: Positive - Presence of IgG antibody to Treponema pallidum detected, suggestive of current or past infection.
Interpretive Data
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are performed in the same laboratory at the same time. The Treponema pallidum Antibody (FTA-ABS), Serum, IgG by IFA (0050477) is recommended for follow-up of reactive nontreponemal tests for syphilis, and as a single test in patients suspected of late syphilis. For CSF specimens, the Treponema pallidum (VDRL) Cerebrospinal Fluid with Reflex to Titer (0050206) test is recommended.
Note
CPT Code(s)
86780
Components
Component Test Code*Component Chart Name
0050920Treponema pallidum Ab, IgG by ELISA
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Cross References
  • Syphilis Antibody, IgG by ELISA
  • T pallidum IgG ELISA