Anti-Neutrophil Cytoplasmic Antibody, IgG
0050811
Ordering Recommendation
 
Mnemonic
ANCA IFA
Methodology
Semi-Quantitative Indirect Fluorescent Antibody
Performed
Mon-Sat
Reported
2-5 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
< 1:20: Not significant  
Interpretive Data
Neutrophil Cytoplasmic Antibodies (C-ANCA=granular cytoplasmic staining, P-ANCA=perinuclear staining) are found in the serum of over 90% of patients with certain necrotizing systemic vasculitides, and usually in less than 5% of patients with collagen vascular disease or arthritis.


 
Note
If the ANCA screen detects antibodies at a 1:20 dilution or greater, then a titer to end point will be added. Additional charges apply. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
CPT Code(s)
86255; if positive, add 86256
Components
Component Test Code*Component Chart Name
0050811Anti-Neutrophil Cytoplasmic Ab, IgG
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ANCA
  • ANCA IgG
  • ANCA IgG Ab