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Anti-Neutrophil Cytoplasmic Antibody, IgG
0050811
Ordering Recommendation

• First-line screening test for ANCA-associated vasculitis.
• A reflex panel is available for the workup of suspected vasculitis; refer to ANCA-Associated Vasculitis Profile (ANCA/MPO/PR-3) Reflex to ANCA Titer (2006480).
• Recommended in the differential evaluation of autoimmune diseases with renal involvement (SLE and anti-glomerular basement membrane disease).
• Recommended adjunct diagnostic tool to differentiate ulcerative colitis from Crohn disease in IBD.

Mnemonic
ANCA IFA
Methodology
Semi-Quantitative Indirect Fluorescent Antibody
Performed
Mon-Sat
Reported
2-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma, urine, or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Less than 1:20: Not significant
Interpretive Data
Neutrophil Cytoplasmic Antibodies (C-ANCA=granular cytoplasmic staining, P-ANCA=perinuclear staining) are found in the serum of over 90% of patients with certain necrotizing systemic vasculitides, and usually in less than 5% of patients with collagen vascular disease or arthritis.





Note
If the ANCA screen detects antibodies at a 1:20 dilution or greater, then a titer to end point will be added. Additional charges apply. ANCA IFA is simultaneously tested on ethanol- and formalin-fixed slides to allow differentiation of C- and P-ANCA patterns.
CPT Code(s)
86255; if positive, add 86256
Components
Component Test Code*Component Chart NameLOINC
0050811Anti-Neutrophil Cytoplasmic Ab, IgG29967-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ANCA
  • ANCA IgG
  • ANCA IgG Ab