Extractable Nuclear Antigen Antibodies (SSA & SSB)
0050791
 
Ordering Recommendation
Mnemonic
SSA/SSB
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
Refer to individual components
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
0050691SSA (Ro) (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-​40 AU/mL: Equivocal
41 AU/mL or greater: Positive
0050692SSB (La) (ENA) Antibody, IgG 29 AU/mL or less: Negative
30-​40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Interpretive Data
SSA (Ro) antibody is seen in 70-75% of Sjögren syndrome cases, 30-40% of systemic lupus erythematosus (SLE) and 5-10% of progressive systemic sclerosis (PSS). SSB (La) antibody is seen in 50-60% of Sjögren syndrome cases and is specific if it is the only ENA antibody present. Fifteen to 25% of patients with systemic lupus erythematosus (SLE) and 5-10% of patients with progressive systemic sclerosis (PSS) also have this antibody.
Note
CPT Code(s)
86235 x2
Components
Component Test Code*Component Chart Name
0050691SSA (Ro) (ENA) Antibody, IgG
0050692SSB (La) (ENA) Antibody, IgG
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Anti-ENA Anti-Ribonuclear Protein
  • Ro Ab
  • Scl 70 Antibodies
  • Smith (ENA) Antibody, IgG
  • SSA (Ro) (ENA) Antibody, IgG
    Anti-SSA
  • SSA Ro
  • SSB (La) (ENA) Antibody, IgG
    Anti-SSB
  • SSB LA