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Extractable Nuclear Antigen Antibodies (SSA 52, SSA 60, and SSB)
0050791
Ordering Recommendation
Differential evaluation of patients with SLE, Sjogren syndrome, polymyositis/dermatomyositis, and/or other tissue diseases or overlapping syndromes.
Mnemonic
SSA/SSB
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma or other body fluids.  Contaminated, hemolyzed, or severely lipemic specimens.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective May 18, 2015
Test Number
Components
Reference Interval
2012074SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
ComponentsReference Interval
SSA 52 (Ro) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA 60 (Ro) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050692SSB (La) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Interpretive Data
SSA (Ro) antibody is seen in 70-75% of Sjögren syndrome cases, 30-40% of systemic lupus erythematosus (SLE) and 5-10% of progressive systemic sclerosis (PSS). SSB (La) antibody is seen in 50-60% of Sjögren syndrome cases and is specific if it is the only ENA antibody present. Fifteen to 25% of patients with systemic lupus erythematosus (SLE) and 5-10% of patients with progressive systemic sclerosis (PSS) also have this antibody.


Note
CPT Code(s)
86235 x3
Components
Component Test Code*Component Chart NameLOINC
0050691SSA 52 (Ro) (ENA) Antibody, IgG31625-7
0050692SSB (La) (ENA) Antibody, IgG29965-1
2012055SSA 60 (Ro) (ENA) Antibody, IgG53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-ENA Anti-Ribonuclear Protein
  • Ro Ab
  • Scl 70 Antibodies
  • Smith (ENA) Antibody, IgG
  • SSA (Ro) (ENA) Antibody, IgG
    Anti-SSA
  • SSA Ro
  • SSB (La) (ENA) Antibody, IgG
    Anti-SSB
  • SSB LA