Diphtheria, Tetanus, and H. Influenzae b Antibodies, IgG
Ordering Recommendation

Evaluate the ability of a patient to produce antibody to pure protein vaccines (diphtheria and tetanus) and protein conjugated bacterial (H. influenza) vaccines after vaccination to rule out antibody deficiency.

Quantitative Multiplex Bead Assay
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Transfer 1.5 mL serum to an ARUP Standard Transport Tube. (Min: 0.45 mL) Acute and convalescent specimens must be labeled as such. Clearly mark specimens as "Pre-Vaccine" or "Post-Vaccine". Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens.  
Storage/Transport Temperature
Unacceptable Conditions
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Diphtheria and tetanus:
Antibody concentration of  > 0.1 IU/mL is usually considered protective for diphtheria or tetanus.

Haemophilus influenzae
type B:
< 1.0 µg/mL = Antibody concentration not protective.
> 1.0 µg/mL = Antibody to H. influenzae type B detected.  Suggestive of protection.
Interpretive Data
Responder status is determined according to the ratio of a one-month, post-vaccination sample to pre-vaccination concentration of IgG antibodies as follows:

Diphtheria and tetanus:
1. If the post-vaccination concentration is less than 1.0 IU/mL, the patient is considered a nonresponder.
2. If the post-vaccination concentration is greater than or equal to 1.0 IU/mL, a patient with a ratio of less than 1.5 is a nonresponder, and a ratio of 1.5 to less than 3.0 is a weak responder, and a ratio of 3.0 or greater is a good responder.
3. If the pre-vaccination concentration is greater than 1.0 IU/mL, it may be difficult to assess the response based on a ratio alone. A post-vaccination concentration above 2.5 IU in this case is usually adequate.

Haemophilus influenza
1. If the post-vaccination concentration is<3.0 µg/mL, the patient is considered to be a nonresponder.
2. If the post-vaccination concentration is 3.0 µg/mL, a patient with a ratio of 4 is a good responder, a ratio of 2-4 is weak responder, and a ratio of<2 is considered a nonresponder.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
86317 x3
Component Test Code*Component Chart NameLOINC
0050210Diphtheria Antibody, IgG13227-4
0050535Tetanus Antibody, IgG6367-7
0050542Haemophilus influenzae b Antibody, IgG11256-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Anti-tetanus toxoid
  • C. tetani
  • Clostridium tetani
  • Corynebacterium diphtheriae Antitoxin
  • Diphtheria Antitoxoid
  • HIB
  • Tetanus Immune Response
  • Tetanus toxoid antibodies
  • Tetanus/Diphtheria Antibody Panel